Cloning of a Mycobacterium tuberculosis gene encoding a purifed protein derivative protein that elicits strong tuberculosis-specific delayed-type hypersensitivity.

Abstract	The purified protein derivative (PPD) skin test has been used for the diagnosis of tuberculosis for more than 75 years. However, the test lacks specificity because all mycobacteria share antigens present in PPD. Therefore, sensitization with nontuberculous pathogenic or with environmental nonpathogenic mycobacteria can lead to positive skin tests. This communication describes a novel PPD protein present only in tuberculous complex mycobacteria. A recombinant protein was obtained and named DPPD on the basis of the first 4 amino acids of its N-terminus sequence. DPPD elicited delayed-type hypersensitivity (DTH) in 100% of Mycobacterium tuberculosis-infected guinea pigs but in no animals sensitized with several organisms representative of all members of the Mycobacterium genus. Preliminary results indicate that DPPD induces strong and specific DTH in humans. This work points to the definition of a single recombinant M. tuberculosis protein that may be an alternative to the PPD test.
Authors	R N Coler, Y A Skeiky, P J Ovendale, T S Vedvick, L Gervassi, J Guderian, S Jen, S G Reed, A Campos-Neto
Journal	The Journal of infectious diseases (J Infect Dis) Vol. 182 Issue 1 Pg. 224-33 (Jul 2000) ISSN: 0022-1899 [Print] United States
PMID	10882601 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Chemical References	Antigens, Bacterial Bacterial Proteins DPPD protein, Mycobacterium tuberculosis Recombinant Proteins Tuberculin Tumor Necrosis Factor Receptor Superfamily, Member 7
Topics	Animals Antigens, Bacterial (genetics, immunology) Bacterial Proteins (genetics, immunology) Cloning, Molecular Disease Models, Animal Guinea Pigs Hypersensitivity, Delayed Immunoblotting Leukocytes, Mononuclear (drug effects, physiology) Mycobacterium tuberculosis (genetics, immunology) Recombinant Proteins (immunology, pharmacology) Tuberculin (pharmacology) Tuberculin Test (methods) Tuberculosis (metabolism, physiopathology) Tumor Necrosis Factor Receptor Superfamily, Member 7 (drug effects, physiology)

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