[Peripheral blood stem cell collection in pediatric oncohematology. Experience with patients weighing less than 15 kg].

Abstract

BACKGROUND:

Leukapheresis for peripheral blood stem cell collection is increasingly being carried out in pediatric cancer patients. Aim of this study was to report experience of the Padua Apheresis Unit on a series of children weighting < 15 kg who have undergone such an apheresis procedure.

METHODS:

This retrospective study includes 15 pediatric patients affected with various malignancies (neuroblastoma: 7; acute myelogenous leukemia: 3; rhabdomyosarcoma: 2; PNET: 1; retinoblastoma: 1; Burkitt's lymphoma: 1) collecting peripheral blood stem cells by a Cobe Spectra blood cell separator. Main procedure parameters, including vascular access, leukapheresis duration, blood flow rate, processed blood volumes, side effects, mononuclear and CD34+ cell yields, have been registered.

RESULTS:

Altogether 22 sessions have been carried out, by processing a mean of 2.8 blood volumes. No leukapheresis related complications have been recorded, such as hypotension, hypocalcemia and hypothermia. Noteworthy, in 4 procedures two or more peripheral venipunctures have been performed to ensure an adequate blood flow.

CONCLUSIONS:

Leukapheresis for peripheral blood stem cell collection can be safely and efficaciously carried out in pediatric patients, even weighing < 15 kg, on the condition that certain aspects of apheresis practice in children (vascular access, volume shifts, anticoagulation, side effects) are carefully considered.

• Authors: P Marson, M G Petris, C Messina, G Cecchetto, R Giordano, L Zanesco, G De Silvestro
• Journal: Minerva pediatrica (Minerva Pediatr) Vol. 52 Issue 3 Pg. 129-35 (Mar 2000) ISSN: 0026-4946 [Print] Italy
• Vernacular Title: Raccolta di cellule staminali periferiche in oncoematologia pediatrica. Esperienza su soggetti di peso inferiore a 15 kg.
• PMID: 10879003 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Antigens, CD34

Topics

• Antibodies, Monoclonal
• Antigens, CD34 (metabolism)
• Body Weight
• Child, Preschool
• Female
• Humans
• Infant
• Leukapheresis (methods)
• Male
• Neoplasms (blood)
• Neoplastic Stem Cells (metabolism)
• Retrospective Studies