Increased high-density lipoprotein-3 binding to leukocytes following weight loss and improved glycemic control in type 2 diabetic patients.

Abstract

This study evaluates the effect on high-density lipoprotein (HDL) binding activity in cultured granulocytes before and after metabolic control of non-insulin-dependent diabetes mellitus ([NIDDM] type 2 diabetes) patients. In 20 type 2 diabetic patients, diabetic control was accomplished by administration of oral antidiabetic agents and dietary restrictions. Adequate metabolic control was reflected by a decrease in the fasting glucose, glycosylated hemoglobin (HbA1c), mean insulin, and body mass index (BMI). After control of the diabetes, the mean HDL3 cholesterol was increased from 0.918 +/- 0.05 to 1.008 +/- 0.05 mmol/L (P < .05) and apolipoprotein AI (apo AI) was increased from 103 +/- 5.8 to 115 +/- 5.1 mg/dL (P < .01). The HDL3 maximum specific binding was higher after versus before diabetic control, 77 +/- 6 versus 122 +/- 8 ng/mg cell protein (P < .01). This increase was related to an increase in maximum binding ([Bmax] from 4.97 x 10(-10) to 8.3 x 10(-10) mol/L, P < .001), and no significant changes were observed in the Kd (from 1.47 x 10(-7) v 2.04 x 10(-7) mol/L). These results suggest that the metabolic control of type 2 diabetes increases HDL3 binding activity.

• Authors: J A Paniagua, J López-Miranda, S Jansen, J L Zambrana, F López Segura, J A Jiménez Perepérez, F Pérez-Jiménez
• Journal: Metabolism: clinical and experimental (Metabolism) Vol. 49 Issue 6 Pg. 692-7 (Jun 2000) ISSN: 0026-0495 [Print] United States
• PMID: 10877191 (Publication Type: Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Blood Glucose
• Carrier Proteins
• Iodine Radioisotopes
• Lipids
• Lipoproteins, HDL
• Lipoproteins, HDL3
• RNA-Binding Proteins
• Receptors, Lipoprotein
• high density lipoprotein receptors
• high density lipoprotein binding protein

Topics

• Adult
• Blood Glucose (metabolism)
• Carrier Proteins
• Diabetes Mellitus, Type 2 (blood, metabolism)
• Female
• Granulocytes (metabolism)
• Humans
• Iodine Radioisotopes
• Lipids (blood)
• Lipoproteins, HDL (blood)
• Lipoproteins, HDL3
• Male
• Middle Aged
• Protein Binding
• RNA-Binding Proteins
• Receptors, Lipoprotein (blood)