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Estradiol-17beta sulfotransferase activity in canine osteosarcoma D17 cells.

Abstract
Estrogen sulfatase and sulfotransferase (EST) activities are present in breast cancer tissues but there are no reports on EST in cancerous bone cells. We incubated [(3)H]estradiol-17beta with cells from a canine osteosarcoma D17 line for periods up to 24 h. Radioactive steroids were recovered from the media and separated into unconjugated and conjugated fractions using Sep-Pak C18 cartridges. The conjugate fraction was solvolyzed and the resulting free steroids were obtained from a second C18 cartridge. Little metabolism was apparent in 4 h of incubation, but by 24 h as much as one half of the radioactivity was seen in the conjugate fraction. Most of the conjugates were recovered as sulfates in all three experiments. HPLC profiles showed a limited metabolism of estradiol to other compounds except for estrone, which was clearly present in both free and sulfate fractions. These results suggest that EST may have a role in the local metabolism of estrogens in bone.
AuthorsJ I Raeside, H L Christie, L Forster, R L Renaud
JournalBiochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 273 Issue 2 Pg. 505-8 (Jul 05 2000) ISSN: 0006-291X [Print] United States
PMID10873635 (Publication Type: Journal Article)
CopyrightCopyright 2000 Academic Press.
Chemical References
  • Estradiol
  • Sulfotransferases
  • estradiol-17 beta sulfotransferase
  • Sulfatases
  • estrogen sulfatase
Topics
  • Animals
  • Bone Neoplasms (enzymology)
  • Bone and Bones (metabolism)
  • Breast Neoplasms (enzymology)
  • Chromatography, High Pressure Liquid
  • Dogs
  • Estradiol (metabolism)
  • Female
  • Humans
  • Osteosarcoma (enzymology)
  • Sulfatases (metabolism)
  • Sulfotransferases (metabolism)
  • Tumor Cells, Cultured

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