Abstract |
Estrogen sulfatase and sulfotransferase (EST) activities are present in breast cancer tissues but there are no reports on EST in cancerous bone cells. We incubated [(3)H] estradiol-17beta with cells from a canine osteosarcoma D17 line for periods up to 24 h. Radioactive steroids were recovered from the media and separated into unconjugated and conjugated fractions using Sep-Pak C18 cartridges. The conjugate fraction was solvolyzed and the resulting free steroids were obtained from a second C18 cartridge. Little metabolism was apparent in 4 h of incubation, but by 24 h as much as one half of the radioactivity was seen in the conjugate fraction. Most of the conjugates were recovered as sulfates in all three experiments. HPLC profiles showed a limited metabolism of estradiol to other compounds except for estrone, which was clearly present in both free and sulfate fractions. These results suggest that EST may have a role in the local metabolism of estrogens in bone.
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Authors | J I Raeside, H L Christie, L Forster, R L Renaud |
Journal | Biochemical and biophysical research communications
(Biochem Biophys Res Commun)
Vol. 273
Issue 2
Pg. 505-8
(Jul 05 2000)
ISSN: 0006-291X [Print] United States |
PMID | 10873635
(Publication Type: Journal Article)
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Copyright | Copyright 2000 Academic Press. |
Chemical References |
- Estradiol
- Sulfotransferases
- estradiol-17 beta sulfotransferase
- Sulfatases
- estrogen sulfatase
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Topics |
- Animals
- Bone Neoplasms
(enzymology)
- Bone and Bones
(metabolism)
- Breast Neoplasms
(enzymology)
- Chromatography, High Pressure Liquid
- Dogs
- Estradiol
(metabolism)
- Female
- Humans
- Osteosarcoma
(enzymology)
- Sulfatases
(metabolism)
- Sulfotransferases
(metabolism)
- Tumor Cells, Cultured
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