Abstract | BACKGROUND: The development and growth of the prostate gland is regulated, in part, by a variety of steroid and polypeptide growth-factor hormones. As a consequence of hormone action, the prostate gland will produce a number of tissue-restricted gene products. Characterization of the regulation, expression, and function of genes encoding prostate-specific proteins is critical to our understanding of prostate biology. Probasin is a prostate-specific gene originally isolated from the rat and has been exploited as a marker of prostate differentiation and to elucidate androgen action. Furthermore, a number of transgenic mouse models of prostate cancer have been established based on the regulatory elements derived from the rat probasin gene. In this report, we describe the isolation and characterization of the mouse probasin ortholog to further facilitate studies related to hormone action in the prostate and the generation and characterization of novel autochthonous models of prostate cancer. METHODS: RESULTS: CONCLUSIONS:
Probasin is a marker of differentiation and androgen action in the mouse prostate, and strong sequence conservation between mouse and rat probasin supports an essential role for this gene in the biology of the prostate gland. Isolation and characterization of mouse probasin will facilitate further development and analysis of autochthonous mouse models of prostate cancer.
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Authors | M A Johnson, I Hernandez, Y Wei, N Greenberg |
Journal | The Prostate
(Prostate)
Vol. 43
Issue 4
Pg. 255-62
(Jun 01 2000)
ISSN: 0270-4137 [Print] United States |
PMID | 10861744
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Copyright | Copyright 2000 Wiley-Liss, Inc. |
Chemical References |
- Androgen-Binding Protein
- DNA, Complementary
- Immune Sera
- probasin
- Transglutaminases
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Topics |
- Amino Acid Sequence
- Androgen-Binding Protein
(isolation & purification)
- Animals
- Base Sequence
- Blotting, Western
- Cell Differentiation
- DNA, Complementary
(chemistry)
- Immune Sera
- Male
- Mice
- Mice, Inbred C57BL
- Molecular Sequence Data
- Polymerase Chain Reaction
- Prostate
(metabolism)
- Rabbits
- Rats
- Transglutaminases
(metabolism)
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