Suppression of N-nitrosomethylbenzylamine-induced rat esophageal tumorigenesis by dietary feeding of auraptene.

Abstract

The modifying effects of auraptene on N-nitrosomethylbenzylamine (NMBA)-induced esophageal tumorigenesis were investigated in male F344 rats. At 5 weeks of age, all animals, except those with the test chemical alone and control rats, received s.c. injections of NMBA (0.5 mg/kg body weight/injection, three times per week) for 5 weeks. At the end of the study (20 weeks), 75% of the rats treated with NMBA alone had esophageal neoplasms (papillomas). However, the groups who received a dose of 500 ppm auraptene during the initiation phase developed significantly reduced incidence of tumors (39%; P<0.05). Exposure to auraptene (500 ppm) during the post-initiation phase also decreased the frequency of the tumors (29%; P<0.01). The reduction of the incidence of severe dysplasia was obtained when auraptene was administered in the post-initiation phase (P<0.05). Cell proliferation in the esophageal epithelium determined by proliferating cell nuclear antigen (PCNA) was lowered by auraptene (P<0.01). Blood polyamine contents in rats who received NMBA and the test compound were also smaller than those of rats that received the carcinogen (P<0.05). These findings suggest that dietary auraptene is effective in inhibiting the development of esophageal tumors by NMBA when given during the initiation as well as post-initiation phases, and such inhibition is related to suppression of cell proliferation in the esophageal epithelium.

• Authors: K Kawabata, T Tanaka, T Yamamoto, A Hara, A Murakami, K Koshimizu, H Ohigashi, G D Stoner, H Mori
• Journal: Journal of experimental & clinical cancer research : CR (J Exp Clin Cancer Res) Vol. 19 Issue 1 Pg. 45-52 (Mar 2000) ISSN: 0392-9078 [Print] England
• PMID: 10840935 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anticarcinogenic Agents
• Carcinogens
• Coumarins
• nitrosobenzylmethylamine
• aurapten
• Dimethylnitrosamine

Topics

• Animals
• Anticarcinogenic Agents (administration & dosage)
• Carcinogens (toxicity)
• Coumarins (administration & dosage)
• Diet
• Dimethylnitrosamine (analogs & derivatives, toxicity)
• Esophageal Neoplasms (chemically induced, prevention & control)
• Incidence
• Male
• Rats
• Rats, Inbred F344