Abstract | BACKGROUND: The basal lipid peroxide concentration in the plasma of patients with hyperlipidemia may be related to atherosclerosis. Quantitative determination of lipid peroxides in the plasma is an important step in the overall evaluation of the biochemical processes leading to oxidative injury. Unfortunately, the currently available methods for lipid peroxidation lack specificity and sensitivity. METHODS: Hyperlipidemic patients (44 males and 50 females), ages 12-82 years (mean +/- SE, 53 +/- 2.3 years for males, 58 +/- 2.0 years for females, and 56 +/- 14 years for total cases), and normolipidemic volunteers (controls, 32 males and 15 females), ages 13-90 years (49 +/- 4 years for males, 65 +/- 4 years for females, and 55 +/- 24 years for total cases), were recruited in the present study. Plasma phosphatidylcholine hydroperoxide ( PCOOH) was determined by chemiluminescence-HPLC (CL-HPLC). RESULTS: CONCLUSIONS: Our results suggest that an increase in plasma PCOOH in patients with hyperlipidemia may be related to the development and progression of atherosclerosis, particularly in the elderly. Measurement of plasma PCOOH is useful for in vivo evaluation of oxidative stress.
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Authors | M Kinoshita, S Oikawa, K Hayasaka, A Sekikawa, T Nagashima, T Toyota, T Miyazawa |
Journal | Clinical chemistry
(Clin Chem)
Vol. 46
Issue 6 Pt 1
Pg. 822-8
(Jun 2000)
ISSN: 0009-9147 [Print] England |
PMID | 10839771
(Publication Type: Journal Article)
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Chemical References |
- Phosphatidylcholines
- phosphatidylcholine hydroperoxide
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Topics |
- Adolescent
- Adult
- Age Factors
- Aged
- Aged, 80 and over
- Female
- Humans
- Hyperlipidemias
(blood)
- Hyperlipoproteinemia Type II
(blood)
- Hyperlipoproteinemia Type IV
(blood)
- Male
- Middle Aged
- Phosphatidylcholines
(blood)
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