Abstract | OBJECT: METHODS: This study was divided into three parts. In the first part, 100 microl of autologous blood was infused into the rats' right basal ganglia, and the animals were killed at 24 and 72 hours after intracerebral infusion. Their brains were tested for complement factors C9, C3d, and clusterin (a naturally occurring complement inhibitor) by using immunohistochemical analysis. In the second part of the study, the rats were killed at 24 or 72 hours after injection of 100 microl of blood. The C9 and clusterin proteins were quantitated using Western blot analysis. In the third part, the rats received either 100 microl of blood or 100 microl of blood plus 10 microg of N-acetylheparin (a complement activation inhibitor). Then they were killed 24 or 72 hours later for measurement of brain water and ion contents. It was demonstrated on Western blot analysis that there had been a sixfold increase in C9 around the hematoma 24 hours after the infusion of 100 microl of autologous blood. Marked perihematomal C9 immunoreactivity was detected at 72 hours. Clusterin also increased after ICH and was expressed in neurons 72 hours later. The addition of N-acetylheparin significantly reduced brain edema formation in the ipsilateral basal ganglia at 24 hours (78.5 +/- 0.5% compared with 81.6 +/- 0.8% in control animals, p < 0.001) and at 72 hours (80.9 +/- 2.2% compared with 83.6 +/- 0.9% in control animals, p < 0.05) after ICH. CONCLUSIONS:
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Authors | Y Hua, G Xi, R F Keep, J T Hoff |
Journal | Journal of neurosurgery
(J Neurosurg)
Vol. 92
Issue 6
Pg. 1016-22
(Jun 2000)
ISSN: 0022-3085 [Print] United States |
PMID | 10839264
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Clusterin
- Complement C9
- Glycoproteins
- Molecular Chaperones
- N-acetylheparin
- Complement C3d
- Heparin
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Topics |
- Animals
- Brain Diseases
(metabolism)
- Brain Edema
(drug therapy, prevention & control)
- Cerebral Hemorrhage
(blood, complications, metabolism)
- Clusterin
- Complement Activation
(drug effects)
- Complement C3d
(metabolism)
- Complement C9
(metabolism)
- Glycoproteins
(metabolism)
- Hematoma
(metabolism)
- Heparin
(analogs & derivatives, therapeutic use)
- Male
- Molecular Chaperones
- Rats
- Rats, Sprague-Dawley
- Up-Regulation
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