Abstract |
The cyanine dye 1,1',3,3,3',3'-hexamethylindodicarbocyanine iodide (HIDC) protects K562 leukemia cells from photodynamic membrane damage caused by cis-di(4-sulfonatophenyl)diphenylporphine (TPPS2) and 420 nm light. This wavelength of light is chosen because it is absorbed by TPPS2, but not by HIDC. The photodynamic system studied may be useful as a model for antineoplastic therapy. A subline of K562 leukemia (K562/DOX), expressing the multidrug-resistance (MDR) phenotype, is found to accumulate smaller amounts of HIDC than the parent cell line and thus has less photoprotection. In the absence of added HIDC, the K562/DOX cell line is more resistant to photodynamic cytotoxicity than the K562 cell line. The resistance of the K562/DOX cell line is not due to a smaller accumulation of TPPS2 than the K562 cell line. However, when both cell lines are incubated with HIDC and TPPS2, and then exposed to light, the K562/DOX cell line becomes more sensitive to photodynamic cell damage than the K562 cell line. The combination of a photosensitizer with a cationic or lysomorphotropic photoprotector represents a novel strategy for the eradication of malignant cells expressing the MDR phenotype.
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Authors | J R Kanofsky, P D Sima |
Journal | Journal of photochemistry and photobiology. B, Biology
(J Photochem Photobiol B)
Vol. 54
Issue 2-3
Pg. 136-44
(Feb 2000)
ISSN: 1011-1344 [Print] Switzerland |
PMID | 10836544
(Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- Coloring Agents
- Cyanides
- Photosensitizing Agents
- Porphyrins
- tetraphenylporphine sulfonate
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Topics |
- Cell Membrane
(drug effects, pathology)
- Coloring Agents
(pharmacology)
- Cyanides
(metabolism, pharmacology)
- Drug Resistance
- Humans
- K562 Cells
- Photosensitizing Agents
(pharmacology)
- Porphyrins
(pharmacology)
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