Arp2/3 complex-independent actin regulatory function of WAVE.

Abstract

We report that WAVE1/Scar1, a WASP-family protein that functions downstream of Rac in membrane ruffling, can induce part of the reorganization of the actin cytoskeleton without Arp2/3 complex. WAVE1 has been reported to associate and activate Arp2/3 complex at its C-terminal region that is rich in acidic residues. The deletion of the acidic residues abolished the interaction with and the activation ability of Arp2/3 complex. The expression of the mutant WAVE1 lacking the acidic residues (DeltaA), however, induced actin-clustering in cells as the wild-type WAVE1 did. In addition, this actin-clustering could not be suppressed by the coexpression of the Arp2/3 complex-sequestering fragment (CA-region) derived from N-WASP, which clearly inhibits Rac-induced membrane ruffling. This study therefore demonstrates that WAVE1 reorganizes the actin cytoskeleton not only through Arp2/3 complex but also through another unidentified mechanism that may be important but has been neglected thus far.

• Authors: N Sasaki, H Miki, T Takenawa
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 272 Issue 2 Pg. 386-90 (Jun 07 2000) ISSN: 0006-291X [Print] United States
• PMID: 10833423 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2000 Academic Press.

Chemical References

• Actin-Related Protein 2
• Actin-Related Protein 3
• Actins
• Actr2 protein, mouse
• Actr3 protein, mouse
• Biopolymers
• Cytoskeletal Proteins
• Microfilament Proteins
• Nerve Tissue Proteins
• Peptide Fragments
• Recombinant Fusion Proteins
• Wasf1 protein, mouse
• Wasl protein, mouse
• Wiskott-Aldrich Syndrome Protein Family
• Wiskott-Aldrich Syndrome Protein, Neuronal

Topics

• 3T3 Cells
• Actin-Related Protein 2
• Actin-Related Protein 3
• Actins (metabolism)
• Amino Acid Sequence
• Animals
• Binding Sites
• Biopolymers (metabolism)
• Cytoskeletal Proteins
• Cytoskeleton (metabolism)
• Hydrogen-Ion Concentration
• Mice
• Microfilament Proteins (chemistry, genetics, metabolism)
• Molecular Sequence Data
• Nerve Tissue Proteins (chemistry, genetics, metabolism)
• Peptide Fragments (chemistry, genetics, metabolism)
• Protein Binding
• Recombinant Fusion Proteins (chemistry, genetics, metabolism)
• Sequence Deletion (genetics)
• Wiskott-Aldrich Syndrome Protein Family
• Wiskott-Aldrich Syndrome Protein, Neuronal