Cytolytic T lymphocytes raised against a human bladder carcinoma recognize an antigen encoded by gene MAGE-A12.

Abstract

Human bladder carcinoma line LB831-BLC expresses several distinct Ags that are recognized by different autologous CTL. Here, we show that one of these Ags is presented by HLA-Cw7 and encoded by gene MAGE-A12. This is the first time that CTL directed against a MAGE-encoded Ag have been derived from the lymphocytes of a patient with cancer other than melanoma. This new Ag was found to be nonapeptide VRIGHLYIL, corresponding to position 170-178 of the MAGE-A12 protein. Gene MAGE-A12 is silent in normal tissues except in male germline cells, which do not express HLA molecules. It is expressed in 26-62% of melanomas, infiltrating bladder carcinomas, lung carcinomas, esophageal carcinomas, and head and neck carcinomas. Because HLA-Cw7 is present in 43% of Caucasians, this new Ag is shared by many tumors and should be a useful target for cancer immunotherapy.

• Authors: L Heidecker, F Brasseur, M Probst-Kepper, M Guéguen, T Boon, B J Van den Eynde
• Journal: Journal of immunology (Baltimore, Md. : 1950) (J Immunol) Vol. 164 Issue 11 Pg. 6041-5 (Jun 01 2000) ISSN: 0022-1767 [Print] United States
• PMID: 10820289 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Neoplasm
• MAGEA12 protein, human
• Neoplasm Proteins
• Peptide Fragments

Topics

• Aged
• Antigen Presentation (genetics)
• Antigens, Neoplasm (biosynthesis, genetics, metabolism)
• Clone Cells
• Esophageal Neoplasms (genetics)
• Genes, Neoplasm (immunology)
• Head and Neck Neoplasms (genetics)
• Humans
• Lung Neoplasms (genetics)
• Male
• Melanoma (genetics)
• Neoplasm Proteins (biosynthesis, genetics, immunology, metabolism)
• Peptide Fragments (genetics, immunology, metabolism)
• T-Lymphocytes, Cytotoxic (immunology, metabolism)
• Tumor Cells, Cultured
• Urinary Bladder Neoplasms (genetics, immunology)