Expression of TRAIL (Apo2L), DR4 (TRAIL receptor 1), DR5 (TRAIL receptor 2) and TRID (TRAIL receptor 3) genes in multidrug resistant human acute myeloid leukemia cell lines that overexpress MDR 1 (HL60/Tax) or MRP (HL60/AR).

Abstract	Previously we have reported a differential expression of CD95/CD95L and Bcl-2 family of genes in multidrug resistant tumor cells. TRAIL, a member of the TNF receptor family, induces apoptosis in many tumor cells by binding to DR4 (TRAIL receptor 1) and DR5 (TRAIL receptor 2). In contrast, TRAIL-induced apoptosis is prevented by a decoy receptor (DcR1, TRID or TRAIL receptor 3). In the present study, we compared the expression of TRAIL, DR4, DR5, and TRID between a drug sensitive HL60, a myeloid leukemia cell line, and its multidrug resistant (MDR) sublines that either overexpressed MDR 1 gene (HL60/Tax) or MRP gene (HL60/AR), using RT-PCR. TRAIL mRNA was expressed in HL60 cells but was present in low levels in HL60/AR cells and was completely lacking in HL60/Tax cells. Both DR4 and DR5 were undetectable in HL60/Tax but were present at comparable levels in HL60/AR and drug sensitive HL60 cells. TRID were absent in HL60 and HL60/Tax cells, but was present in low but comparable levels in peripheral blood mononuclear cells and HL60/AR cells. These data suggest that the multidrug resistance in MDR HL60 cell lines, regardless of overexpression of MDR 1 or MRP, may be due to different mechanisms. In HL60/AR cells it appears that MDR may be due to decreased expression of TRAIL and constitutive expression of TRID, whereas in HL60/Tax cells, MDR could be due to the absence of TRAIL and/or DR4 and DR5.
Authors	C H Kim, S Gupta
Journal	International journal of oncology (Int J Oncol) Vol. 16 Issue 6 Pg. 1137-9 (Jun 2000) ISSN: 1019-6439 [Print] Greece
PMID	10811986 (Publication Type: Journal Article)
Chemical References	ATP-Binding Cassette Transporters Apoptosis Regulatory Proteins Fungal Proteins GPI-Linked Proteins MDR1 protein, Filobasidiella neoformans Membrane Glycoproteins Multidrug Resistance-Associated Proteins Neoplasm Proteins RNA, Messenger Receptors, TNF-Related Apoptosis-Inducing Ligand Receptors, Tumor Necrosis Factor Receptors, Tumor Necrosis Factor, Member 10c TNF-Related Apoptosis-Inducing Ligand TNFRSF10A protein, human TNFRSF10B protein, human TNFRSF10C protein, human TNFSF10 protein, human Tumor Necrosis Factor Decoy Receptors Tumor Necrosis Factor-alpha
Topics	ATP-Binding Cassette Transporters (metabolism) Apoptosis Regulatory Proteins Drug Resistance, Multiple Drug Resistance, Neoplasm Fungal Proteins GPI-Linked Proteins HL-60 Cells (metabolism) Humans Membrane Glycoproteins (metabolism) Multidrug Resistance-Associated Proteins Neoplasm Proteins (metabolism) RNA, Messenger (metabolism) Receptors, TNF-Related Apoptosis-Inducing Ligand Receptors, Tumor Necrosis Factor (metabolism) Receptors, Tumor Necrosis Factor, Member 10c TNF-Related Apoptosis-Inducing Ligand Tumor Necrosis Factor Decoy Receptors Tumor Necrosis Factor-alpha (metabolism)

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