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Comparison of assays for anti-endomysial and anti-transglutaminase antibodies for diagnosis of pediatric celiac disease.

AbstractBACKGROUND: Anti-endomysial antibodies are sensitive and specific markers for celiac disease. This antibody has recently been identified as an antibody to tissue transglutaminase, an enzyme that cross-links and stabilizes extracellular matrix proteins. OBJECTIVES: To evaluate the clinical usefulness of an enzyme-linked immunoassay for anti-transglutaminase antibodies, and to compare the results with those of AEA, the current gold standard serological test for celiac disease. METHODS: Serum samples were collected from 33 patients with biopsy-proven celiac disease and AEA tests were performed. Control samples for anti-transglutaminase were obtained from 155 patients. An ELISA test for immunoglobulin A anti-transglutaminase utilizing guinea pig liver transglutaminase was developed and performed on all sera. Cutoff values for the test were performed using logistic regression and receiver operating curves analysis. RESULTS: An optical density cutoff value of 0.34 was established for the assay. The mean value was 0.18 +/- 0.19 optical density for controls, and 1.65 +/- 1.14 for patients with celiac disease (P < 0.001). Sensitivity and specificity of the assay were both 90%, while AEA had a sensitivity and specificity of 100% and 94%, respectively. CONCLUSIONS: A tissue transglutaminase-based ELISA test is both sensitive and specific for detection of celiac disease.
AuthorsA Levine, Y Bujanover, S Reif, S Gass, N Vardinon, R Reifen, D Lehmann (Affiliation: Department of Pediatrics, Wolfson Medical Center, Holon, Israel. levinemd at netmedia.net.il)
JournalThe Israel Medical Association journal : IMAJ (Isr Med Assoc J) Vol. 2 Issue 2 Pg. 122-5 (Feb 2000) ISSN: 1565-1088 [Print] ISRAEL
PMID10804933 (Publication Type: Comparative Study, Journal Article)
Chemical References
  • Autoantibodies
  • Biological Markers
  • Gliadin
  • Transglutaminases
Topics
  • Analysis of Variance
  • Autoantibodies (immunology)
  • Biological Markers
  • Case-Control Studies
  • Celiac Disease (diagnosis)
  • Enzyme-Linked Immunosorbent Assay (methods)
  • Gliadin (immunology)
  • Humans
  • Logistic Models
  • ROC Curve
  • Sensitivity and Specificity
  • Transglutaminases (immunology)

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