Abstract | BACKGROUND: METHODS: We obtained genomic DNA samples from 227 Japanese men with various drinking habits. ALDH2 genotypes were determined by allele-specific polymerase chain reaction. GGT, CDT, and MCV were determined and compared between ALDH2(1)/2(1) and ALDH2(1)/2(2) habitual drinkers who consumed more than 66 g of alcohol per day for more than 5 years. We measured CDT by anion-exchange chromatography followed by turbidity immunoassay by using a commercially available assay kit (Axis %CDT TIA). RESULTS: CDT levels were comparable between the two groups. GGT activities were significantly greater in ALDH2(1)/2(1) than in ALDH2(1)/2(2) habitual drinkers (81 +/- 85 vs. 53 +/- 40 IU/liters, p < 0.02). MCV values, on the other hand, were significantly larger in ALDH2(1)/2(2) than in ALDH2(1)/2(1) subjects (98.2 +/- 5.8 vs. 95.8 +/- 4.2 fl, p = 0.02). When we used elevation of either CDT or GGT to detect habitual drinking in ALDH2(1)/2(1) and 2(1)/2(2) subjects, the sensitivities were 57% and 46%, respectively. CDT levels were similar between habitual drinkers with normal aspartate aminotransferase levels and those with elevated levels. CONCLUSION: GGT and MCV, but not CDT, differ with respect to the ALDH2 genotypes in Japanese male habitual drinkers. ALDH2 genotypes should be considered when interpreting data on biological markers of alcoholism.
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Authors | F Nomura, S Itoga, M Tamura, S Harada, Y Iizuka, T Nakai |
Journal | Alcoholism, clinical and experimental research
(Alcohol Clin Exp Res)
Vol. 24
Issue 4 Suppl
Pg. 30S-33S
(Apr 2000)
ISSN: 0145-6008 [Print] England |
PMID | 10803776
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers
- Transferrin
- carbohydrate-deficient transferrin
- ALDH2 protein, human
- Aldehyde Dehydrogenase
- Aldehyde Dehydrogenase, Mitochondrial
- gamma-Glutamyltransferase
- Aspartate Aminotransferases
- Alanine Transaminase
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Topics |
- Adult
- Aged
- Aged, 80 and over
- Alanine Transaminase
(blood)
- Alcoholism
(blood, genetics)
- Aldehyde Dehydrogenase
(genetics)
- Aldehyde Dehydrogenase, Mitochondrial
- Aspartate Aminotransferases
(blood)
- Biomarkers
(blood)
- Erythrocyte Indices
- Genotype
- Humans
- Japan
- Male
- Middle Aged
- Polymerase Chain Reaction
- Transferrin
(analogs & derivatives, analysis)
- gamma-Glutamyltransferase
(blood)
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