Secretory leukocyte protease inhibitor inhibits infection of monocytes and lymphocytes with human immunodeficiency virus type 1 but does not interfere with transcytosis of cell-associated virus across tight epithelial barriers.

Abstract

In the present study, we demonstrate that recombinant human secretory leukocyte protease inhibitor (rhSLPI) inhibits infection of lymphocyte- and monocyte-derived tumor cell lines and peripheral blood lymphocytes with laboratory-adapted isolates and with the primary isolate, NDK, of free human immunodeficiency virus type 1 (HIV-1). In contrast, rhSLPI did not exhibit inhibitory activity toward transcytosis of cell-associated HIV-1 through a tight monolayer of endometrial epithelial cells. These observations indicate that the inhibitory effect of SLPI is restricted to free HIV-1 in corporal fluids.

• Authors: H Hocini, P Becquart, H Bouhlal, H Adle-Biassette, M D Kazatchkine, L Bélec
• Journal: Clinical and diagnostic laboratory immunology (Clin Diagn Lab Immunol) Vol. 7 Issue 3 Pg. 515-8 (May 2000) ISSN: 1071-412X [Print] United States
• PMID: 10799472 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Proteinase Inhibitory Proteins, Secretory
• Proteins
• RNA, Messenger
• Recombinant Proteins
• SLPI protein, human
• Secretory Leukocyte Peptidase Inhibitor
• Serine Proteinase Inhibitors

Topics

• Acquired Immunodeficiency Syndrome (immunology, prevention & control, transmission)
• Cervix Uteri (cytology, virology)
• Epithelial Cells (drug effects, metabolism, virology)
• Female
• Gene Expression (immunology)
• HIV Seronegativity
• HIV-1
• Humans
• Lymphocytes (drug effects, metabolism, virology)
• Monocytes (drug effects, metabolism, virology)
• Proteinase Inhibitory Proteins, Secretory
• Proteins (genetics, immunology, pharmacology)
• RNA, Messenger (analysis)
• Recombinant Proteins (pharmacology)
• Secretory Leukocyte Peptidase Inhibitor
• Serine Proteinase Inhibitors (immunology, pharmacology)
• Tight Junctions (metabolism, virology)