Experimental induction of apoptosis by a combination of etoposide and radiation treatment.

Abstract	The present study was undertaken to investigate cell death (particularly apoptosis) induced by etoposide, radiation, and both, and to examine p53 protein expression in relation to cell death. Nude mice transplanted with a human tumor (ependymoblastoma) were treated with etoposide (5-40 mg/kg) or 1-2 Gy X-ray irradiation or both. The tumor was excised at different points after treatment, and tumor tissue specimens were used to check for apoptosis and p53 protein expression by TUNEL, p53 protein staining, etc. Induction of p53-dependent apoptosis was observed in the etoposide treatment group, the X-ray irradiation group, and the combined (etoposide + X-ray irradiation) group. Etoposide 10 mg/kg was found to be approximately equivalent to 1 Gy X-ray irradiation in ability to induce apoptosis. When etoposide treatment was combined with X-ray irradiation at intervals of 3-6 hours, an approximately additive effect was observed.
Authors	H Toda, M Hasegawa, K Hayakawa, M Kawashima, Y Nakamura, M Yamakawa, N Mitsuhashi, H Niibe
Journal	Anticancer research (Anticancer Res) 2000 Jan-Feb Vol. 20 Issue 1A Pg. 165-70 ISSN: 0250-7005 [Print] Greece
PMID	10769650 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Antineoplastic Agents, Phytogenic DNA, Neoplasm Enzyme Inhibitors Neoplasm Proteins Topoisomerase II Inhibitors Tumor Suppressor Protein p53 Etoposide
Topics	Animals Antineoplastic Agents, Phytogenic (pharmacology) Apoptosis (drug effects, radiation effects) Brain Neoplasms (pathology) DNA Damage DNA, Neoplasm (drug effects, radiation effects) Enzyme Inhibitors (pharmacology) Etoposide (pharmacology) Humans Mice Mice, Inbred BALB C Mice, Nude Neoplasm Proteins (antagonists & inhibitors, biosynthesis) Neoplasm Transplantation Neuroectodermal Tumors, Primitive (pathology) Specific Pathogen-Free Organisms Topoisomerase II Inhibitors Tumor Cells, Cultured (transplantation) Tumor Suppressor Protein p53 (biosynthesis)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.

Realize the full power of the drug-disease research graph!