Abstract | BACKGROUND: METHODS:
Colorectal cancers and adjacent normal mucosa from 21 patients were extracted for protein. Expression levels and activity of the MAPKs (ERK1/2, JNK1, p38 and ERK3) were assessed by immunoblot analysis and in vitro kinase assays, respectively. In addition, changes in myelin basic protein (MBP) kinase activity and autophosphorylation were determined by in-gel kinase assays. RESULTS: The activities of ERK1/2, JNK1 and p38 were downregulated in the majority of cancers; ERK3 kinase activity was increased in 10 of 21 cancers. The presence of proteins displaying increased MBP phosphorylation and autophosphorylation was identified specifically in the cancers by in-gel kinase assays. CONCLUSIONS: Our findings demonstrate that the constitutive activation of ERK1/2, JNK1 and p38 is not a feature of colorectal cancers. Moreover, our in-gel kinase results suggest that protein kinases, other than the MAPKs assessed, may play a more crucial role in colon carcinogenesis.
|
Authors | Q Wang, Q Ding, Z Dong, R A Ehlers, B M Evers |
Journal | Anticancer research
(Anticancer Res)
2000 Jan-Feb
Vol. 20
Issue 1A
Pg. 75-83
ISSN: 0250-7005 [Print] Greece |
PMID | 10769637
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
|
Chemical References |
- Neoplasm Proteins
- Calcium-Calmodulin-Dependent Protein Kinases
- JNK Mitogen-Activated Protein Kinases
- Mitogen-Activated Protein Kinase 1
- Mitogen-Activated Protein Kinase 3
- Mitogen-Activated Protein Kinase 6
- Mitogen-Activated Protein Kinases
- p38 Mitogen-Activated Protein Kinases
- Glycogen Synthase Kinase 3
|
Topics |
- Adenocarcinoma
(enzymology, genetics, pathology)
- Calcium-Calmodulin-Dependent Protein Kinases
(biosynthesis, genetics)
- Colonic Neoplasms
(enzymology, genetics, pathology)
- Gene Expression Regulation, Enzymologic
- Gene Expression Regulation, Neoplastic
- Glycogen Synthase Kinase 3
- Humans
- Intestinal Mucosa
(enzymology)
- JNK Mitogen-Activated Protein Kinases
- MAP Kinase Signaling System
- Mitogen-Activated Protein Kinase 1
(biosynthesis, genetics)
- Mitogen-Activated Protein Kinase 3
- Mitogen-Activated Protein Kinase 6
- Mitogen-Activated Protein Kinases
(biosynthesis, genetics)
- Neoplasm Proteins
(biosynthesis, genetics)
- Neoplasm Staging
- p38 Mitogen-Activated Protein Kinases
|