Decreased expression of corticotropin-releasing factor-binding protein mRNA in ACTH-secreting pituitary adenomas.

Abstract

This study was designed to analyze the sequence and the expression of CRF-BP mRNA in ACTH-secreting pituitary adenomas. Direct sequencing analysis revealed no apparent mutations in the CRF-BP mRNA. Thus, we conclude that mutations in the coding region of the CRF-BP gene are not involved in the pathogenesis of Cushing's disease. However, using a semiquantitative PCR approach coamplifying the house-keeping gene GAPDH we detected a reduced expression of CRF-BP mRNA in ACTH-secreting pituitary adenomas when compared with normal pituitaries. We suggest that the decreased CRF-BP gene expression in ACTH-secreting pituitary adenomas is most likely an effect due to high cortisol levels in Cushing patients.

• Authors: K D Dieterich, H Lehnert
• Journal: Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association (Exp Clin Endocrinol Diabetes) Vol. 108 Issue 1 Pg. 59-62 ( 2000) ISSN: 0947-7349 [Print] Germany
• PMID: 10768834 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Carrier Proteins
• RNA, Messenger
• corticotropin releasing factor-binding protein
• Adrenocorticotropic Hormone

Topics

• Adenoma (metabolism)
• Adrenocorticotropic Hormone (metabolism)
• Adult
• Aged
• Aged, 80 and over
• Carrier Proteins (genetics)
• Female
• Gene Expression
• Humans
• Male
• Middle Aged
• Mutation
• Pituitary Neoplasms (metabolism)
• RNA, Messenger (analysis)
• Sequence Analysis, DNA