Abstract |
MuMig (monokine induced by gamma interferon) and Crg-2 ( cytokine responsive gene) are chemokines of the CXC subfamily. They share activity as T and NK cell chemoattractants. Crg-2 has been shown to be inducible by IFN, TNF, IL-1 and LPS, whereas the expression of MuMig is thought to be strictly dependent on IFN-gamma. In the present study, the kinetics of expression of the genes for MuMig and Crg-2 were analysed by northern blot analysis in organs of normal mice and in groups of gene knockout mice deficient in IFN-gamma (IFN-gamma-/-) or receptors for IFN-alpha/beta and IFN-gamma (double R-/-; DR-/-) after vaccinia virus infection. MuMig mRNA was not expressed in IFN-gamma-/- mice in all organs examined, whereas Crg-2 mRNA levels were marginally reduced. In contrast, MuMig and Crg-2 mRNA transcripts were completely abolished in the DR-/- mice.
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Authors | S Mahalingam, G Karupiah |
Journal | Immunology and cell biology
(Immunol Cell Biol)
Vol. 78
Issue 2
Pg. 156-60
(Apr 2000)
ISSN: 0818-9641 [Print] United States |
PMID | 10762416
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Chemokine CXCL10
- Chemokine CXCL9
- Chemokines, CXC
- Cxcl10 protein, mouse
- Cxcl9 protein, mouse
- Monokines
- RNA, Messenger
- Receptors, Interferon
- Interferon-gamma
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Topics |
- Animals
- Blotting, Northern
- Chemokine CXCL10
- Chemokine CXCL9
- Chemokines, CXC
(metabolism)
- Female
- Interferon-gamma
(deficiency)
- Kinetics
- Liver
(metabolism)
- Mice
- Mice, Inbred C57BL
- Mice, Knockout
- Monokines
(metabolism)
- Ovary
(metabolism)
- RNA, Messenger
(analysis)
- Receptors, Interferon
(deficiency)
- Spleen
(metabolism)
- Uterus
(metabolism)
- Vaccinia
(metabolism, virology)
- Vaccinia virus
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