Abstract |
A gradient high-performance liquid chromatographic (HPLC) method is described for the quantification of KW-2149 and its two major metabolites in plasma. The method involves a sample clean-up by solid-phase extraction on C18 columns, separation of the respective compounds by HPLC on a YMC ODS-AQ column (5-microm particle size, 150x6 mm I.D.), using a methanol-water gradient system as an eluent, and measurement by UV absorbance detection at 375 nm. The limits of quantitation were 10 ng/ml for KW-2149 and M-16, and 15 ng/ml for M-18. Recoveries from plasma were higher than 92% on C18 extraction columns. Intra-day precision, expressed as %C.V., was between 1.4 and 6.5%. Intra-day accuracy ranged from 94 to 107%. Precision and accuracy of variability of inter-assays increased somewhat; however, were still within acceptable ranges. The ability of the method to quantify KW-2149 and two major metabolites simultaneously, with precision, accuracy and sensitivity, make it useful in monitoring the fate of this new mitomycin in cancer patients.
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Authors | Z D Zhang, G Guetens, G De Boeck, K Van Cauwenberghe, R A Maes, C Ardiet, A T van Oosterom, M Highley, E A de Bruijn, U R Tjaden |
Journal | Journal of chromatography. B, Biomedical sciences and applications
(J Chromatogr B Biomed Sci Appl)
Vol. 739
Issue 2
Pg. 281-9
(Mar 10 2000)
ISSN: 1387-2273 [Print] Netherlands |
PMID | 10755372
(Publication Type: Journal Article)
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Chemical References |
- Antibiotics, Antineoplastic
- Mitomycins
- KW 2149
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Topics |
- Antibiotics, Antineoplastic
(blood, pharmacokinetics)
- Chromatography, High Pressure Liquid
(methods)
- Humans
- Mitomycins
(blood, pharmacokinetics)
- Reproducibility of Results
- Sensitivity and Specificity
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