Simultaneous determination of the peptide-mitomycin KW-2149 and its metabolites in plasma by high-performance liquid chromatography.

Abstract

A gradient high-performance liquid chromatographic (HPLC) method is described for the quantification of KW-2149 and its two major metabolites in plasma. The method involves a sample clean-up by solid-phase extraction on C18 columns, separation of the respective compounds by HPLC on a YMC ODS-AQ column (5-microm particle size, 150x6 mm I.D.), using a methanol-water gradient system as an eluent, and measurement by UV absorbance detection at 375 nm. The limits of quantitation were 10 ng/ml for KW-2149 and M-16, and 15 ng/ml for M-18. Recoveries from plasma were higher than 92% on C18 extraction columns. Intra-day precision, expressed as %C.V., was between 1.4 and 6.5%. Intra-day accuracy ranged from 94 to 107%. Precision and accuracy of variability of inter-assays increased somewhat; however, were still within acceptable ranges. The ability of the method to quantify KW-2149 and two major metabolites simultaneously, with precision, accuracy and sensitivity, make it useful in monitoring the fate of this new mitomycin in cancer patients.

• Authors: Z D Zhang, G Guetens, G De Boeck, K Van Cauwenberghe, R A Maes, C Ardiet, A T van Oosterom, M Highley, E A de Bruijn, U R Tjaden
• Journal: Journal of chromatography. B, Biomedical sciences and applications (J Chromatogr B Biomed Sci Appl) Vol. 739 Issue 2 Pg. 281-9 (Mar 10 2000) ISSN: 1387-2273 [Print] Netherlands
• PMID: 10755372 (Publication Type: Journal Article)

Chemical References

• Antibiotics, Antineoplastic
• Mitomycins
• KW 2149

Topics

• Antibiotics, Antineoplastic (blood, pharmacokinetics)
• Chromatography, High Pressure Liquid (methods)
• Humans
• Mitomycins (blood, pharmacokinetics)
• Reproducibility of Results
• Sensitivity and Specificity