Abstract | OBJECTIVE: BACKGROUND: The specificity of oligoclonal IgG in MS CSF and plaques, and their relevance to the pathogenesis of MS is unknown. METHODS: Nested PCR was used to amplify and sequence the rearranged IgG heavy-chain VH repertoire in plaques of three acute MS brains and in three SSPE brains. A representative population of VH sequences from each tissue was aligned to the known 51 functional VH germline segments. From this the authors determined the closest VH family germline segment, and the degree and location of somatic mutations for each unique IgG. RESULTS: As expected for an antigen-driven response against MV antigens, most VH sequences from the SSPE brains were mutated extensively compared with their closest germline segments. Furthermore, SSPE VH sequences accumulated replacement mutations preferentially in the complementary-determining regions (CDRs) relative to framework regions-features normally observed during antigen-driven selection. A comparison of VH family and germline usage also demonstrated that each SSPE brain had its own unique IgG response. When the authors compared the VH response in MS plaques with SSPE, MS VH sequences were also mutated extensively, displayed a preferential accumulation of replacement mutations in CDRs, and were unique in each MS brain. CONCLUSION: The presence of an antigen-driven response in MS, rather than a nonconventional mechanism of B-cell activation, warrants additional analysis of the specificity of IgG in MS brain and CSF.
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Authors | T Smith-Jensen, M P Burgoon, J Anthony, H Kraus, D H Gilden, G P Owens |
Journal | Neurology
(Neurology)
Vol. 54
Issue 6
Pg. 1227-32
(Mar 28 2000)
ISSN: 0028-3878 [Print] United States |
PMID | 10746589
(Publication Type: Comparative Study, Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Immunoglobulin G
- RNA Probes
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Topics |
- Adolescent
- Adult
- Blotting, Northern
- Brain
(immunology, pathology)
- Female
- Gene Library
- Humans
- Immunoglobulin G
(genetics, immunology)
- Male
- Multiple Sclerosis
(genetics, immunology, pathology)
- RNA Probes
- Subacute Sclerosing Panencephalitis
(genetics, immunology, pathology)
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