Immunohistochemical expression of inducible nitric oxide synthase in DMBA-induced hamster buccal pouch carcinogenesis.

Abstract

Nitric oxide (NO) plays a key role in the processes of inflammation and carcinogenesis. Three isoforms of NO synthase have been identified: endothelial nitric oxide synthase (NOS), neuronal NOS, and inducible NOS (iNOS). The purpose of this study was to investigate the characteristics of iNOS in 7, 12-dimethylbenz[a]anthracene (DMBA)-induced hamster buccal pouch carcinogenesis. Thirty outbred young (6-week-old) male Syrian golden hamsters were randomly divided into three groups: DMBA (0.5%) painted group (n=10); mineral oil-treated group (n=10); and non-treated group (n=10). The average number of iNOS positive foci per section in the DMBA-treated group was approximately 12.2+/-4.7. Both cytoplasmic and nuclear stainings were observed in the DMBA-treated pouch keratinocytes. No iNOS activity could be detected in the untreated or mineral oil-treated pouches. In conclusion, this study has demonstrated that iNOS is expressed in DMBA-induced hamster pouch carcinomas. This finding suggests that iNOS expression may be associated with the development of chemically induced oral carcinomas.

• Authors: Y K Chen, L M Lin
• Journal: Oral oncology (Oral Oncol) Vol. 36 Issue 2 Pg. 221-4 (Mar 2000) ISSN: 1368-8375 [Print] England
• PMID: 10745176 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Carcinogens
• 9,10-Dimethyl-1,2-benzanthracene
• Nitric Oxide Synthase
• Nitric Oxide Synthase Type II

Topics

• 9,10-Dimethyl-1,2-benzanthracene
• Animals
• Carcinogens
• Cricetinae
• Immunohistochemistry
• Male
• Mesocricetus
• Mouth Neoplasms (chemically induced, enzymology)
• Neoplasms, Experimental
• Nitric Oxide Synthase (metabolism)
• Nitric Oxide Synthase Type II