From androgen receptor to the general transcription factor TFIIH. Identification of cdk activating kinase (CAK) as an androgen receptor NH(2)-terminal associated coactivator.

Abstract

The androgen receptor (AR), like other steroid receptors, modulates the activity of the general transcription machinery on the core promoter to exert its function as a regulator. Co-immunoprecipitation of prostate cancer LNCaP cell extract using protein A-Sepharose coupled with anti-AR antibody indicates that the AR interacts with the general transcription factor TFIIH in a physiological condition. Co-transfection of cdk activating kinase (CAK), the kinase moiety of TFIIH, enhanced AR-mediated transcription in a ligand-dependent manner in human prostate cancer PC-3 and LNCaP cells, and in a ligand-independent manner in human prostate cancer DU145 cells. Detailed interaction studies further revealed that the AR NH(2)-terminal domain interacting with CAK was essential for the CAK-induced AR transactivation. Together, our data suggest that the AR may interact with TFIIH for efficient communication with the general transcription factors/RNA polymerase II on the core promoter.

• Authors: D K Lee, H O Duan, C Chang
• Journal: The Journal of biological chemistry (J Biol Chem) Vol. 275 Issue 13 Pg. 9308-13 (Mar 31 2000) ISSN: 0021-9258 [Print] United States
• PMID: 10734072 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• CCNH protein, human
• Cyclin H
• Cyclins
• DNA-Binding Proteins
• Fungal Proteins
• GAL4 protein, S cerevisiae
• Receptors, Androgen
• Recombinant Fusion Proteins
• Saccharomyces cerevisiae Proteins
• TAF6 protein, S cerevisiae
• TATA-Binding Protein Associated Factors
• Transcription Factor TFIID
• Transcription Factors
• Transcription Factors, TFII
• Transcription Factor TFIIH
• Protein Serine-Threonine Kinases
• Cyclin-Dependent Kinases
• Cyclin-Dependent Kinase-Activating Kinase

Topics

• Cyclin H
• Cyclin-Dependent Kinases
• Cyclins (metabolism)
• DNA-Binding Proteins
• Fungal Proteins (genetics, metabolism)
• Humans
• Male
• Prostatic Neoplasms (metabolism, pathology)
• Protein Serine-Threonine Kinases (chemistry, metabolism)
• Receptors, Androgen (metabolism)
• Recombinant Fusion Proteins (genetics, metabolism)
• Saccharomyces cerevisiae Proteins
• TATA-Binding Protein Associated Factors
• Transcription Factor TFIID
• Transcription Factor TFIIH
• Transcription Factors (genetics, metabolism)
• Transcription Factors, TFII
• Tumor Cells, Cultured
• Cyclin-Dependent Kinase-Activating Kinase