Abstract | BACKGROUND & AIMS: Activated pancreatic stellate cells have recently been implicated in pancreatic fibrogenesis. This study examined the role of pancreatic stellate cells in alcoholic pancreatic fibrosis by determining whether these cells are activated by ethanol itself and, if so, whether such activation is caused by the metabolism of ethanol to acetaldehyde and/or the generation of oxidant stress within the cells. METHODS: RESULTS: Exposure to ethanol or acetaldehyde led to cell activation and intracellular lipid peroxidation. These changes were prevented by the antioxidant vitamin E. Stellate cells exhibited ethanol-inducible ADH activity. Inhibition of ADH by 4MP prevented ethanol-induced cell activation. CONCLUSIONS: Pancreatic stellate cells are activated on exposure to ethanol. This effect of ethanol is most likely mediated by its metabolism (via ADH) to acetaldehyde and the generation of oxidant stress within the cells.
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Authors | M V Apte, P A Phillips, R G Fahmy, S J Darby, S C Rodgers, G W McCaughan, M A Korsten, R C Pirola, D Naidoo, J S Wilson |
Journal | Gastroenterology
(Gastroenterology)
Vol. 118
Issue 4
Pg. 780-94
(Apr 2000)
ISSN: 0016-5085 [Print] United States |
PMID | 10734030
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Actins
- Drug Combinations
- Ferric Compounds
- Lipid Peroxides
- ferric sulfate
- Ethanol
- Malondialdehyde
- Collagen
- Acetaldehyde
- Ascorbic Acid
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Topics |
- Acetaldehyde
(pharmacology)
- Actins
(metabolism)
- Animals
- Ascorbic Acid
(pharmacology)
- Cell Division
(drug effects)
- Cells, Cultured
- Collagen
(biosynthesis)
- Drug Combinations
- Ethanol
(metabolism, pharmacology)
- Ferric Compounds
(pharmacology)
- Fibrosis
- Lipid Peroxides
(metabolism)
- Malondialdehyde
(metabolism)
- Muscle, Smooth
(metabolism)
- Oxidative Stress
- Pancreas
(drug effects, metabolism, pathology)
- Rats
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