Abstract |
Group 2 allergens are a major cause of sensitization in patients allergic to house dust mites. This study was performed to determine the prevalence of hypersensitivity to group 2 allergens ( Der p 2) of Dermatophagoides pteronyssinus (Dp) in asthmatic patients in Taiwan. To facilitate the analysis of Der p 2-specific IgE, we raised a panel of monoclonal antibodies (MoAbs) to Der p 2 antigens. Purified Der p 2 was obtained after MoAb affinity column purification. There were 82 asthmatic patients (41 adults and 41 children) with hypersensitivity to Dp who were analyzed for hypersensitivity to Der p 2. All of them were both skin test- and serology test-reactive to Dp. Using purified Der p 2, 87.8% (72/82) of patients had a skin-test-positive reaction. Six adults (6/41) and 4 children (4/41) had negative skin tests for Der p 2. Ten families (both parents and children were asthmatics) of the 82 patients were selected for Der p 2 skin testing and Der p 2-specific IgE determination using immunoblot analysis. Results showed that 90% (18/20) of patients' skin reactions to Der p 2 and serum contained specific IgE to Der p 2. Because 87.8% (85.4% of adults and 90.2% of children) of the asthmatic patients with Dp hypersensitivity were allergic to Der p 2, its role in the pathogenesis of asthma in Taiwan appears to be important. Purified Der p 2 allergens can be further used for allergen skin testing and immunotherapy.
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Authors | J J Tsai, H D Shen, K Y Chua |
Journal | International archives of allergy and immunology
(Int Arch Allergy Immunol)
Vol. 121
Issue 3
Pg. 205-10
(Mar 2000)
ISSN: 1018-2438 [Print] Switzerland |
PMID | 10729778
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2000 S. Karger AG, Basel |
Chemical References |
- Allergens
- Antibodies, Monoclonal
- Antigens, Dermatophagoides
- Glycoproteins
- Immunoglobulin E
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Topics |
- Adolescent
- Adult
- Allergens
(immunology, isolation & purification, metabolism)
- Antibodies, Monoclonal
(biosynthesis, chemistry)
- Antibody Specificity
- Antigens, Dermatophagoides
- Asthma
(epidemiology, immunology)
- Child
- Female
- Glycoproteins
(immunology, isolation & purification, metabolism)
- Humans
- Immunoglobulin E
(blood, metabolism)
- Male
- Prevalence
- Singapore
(epidemiology)
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