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Purification of group 2 Dermatophagoides pteronyssinus allergen and prevalence of its specific IgE in asthmatics.

Abstract
Group 2 allergens are a major cause of sensitization in patients allergic to house dust mites. This study was performed to determine the prevalence of hypersensitivity to group 2 allergens (Der p 2) of Dermatophagoides pteronyssinus (Dp) in asthmatic patients in Taiwan. To facilitate the analysis of Der p 2-specific IgE, we raised a panel of monoclonal antibodies (MoAbs) to Der p 2 antigens. Purified Der p 2 was obtained after MoAb affinity column purification. There were 82 asthmatic patients (41 adults and 41 children) with hypersensitivity to Dp who were analyzed for hypersensitivity to Der p 2. All of them were both skin test- and serology test-reactive to Dp. Using purified Der p 2, 87.8% (72/82) of patients had a skin-test-positive reaction. Six adults (6/41) and 4 children (4/41) had negative skin tests for Der p 2. Ten families (both parents and children were asthmatics) of the 82 patients were selected for Der p 2 skin testing and Der p 2-specific IgE determination using immunoblot analysis. Results showed that 90% (18/20) of patients' skin reactions to Der p 2 and serum contained specific IgE to Der p 2. Because 87.8% (85.4% of adults and 90.2% of children) of the asthmatic patients with Dp hypersensitivity were allergic to Der p 2, its role in the pathogenesis of asthma in Taiwan appears to be important. Purified Der p 2 allergens can be further used for allergen skin testing and immunotherapy.
AuthorsJ J Tsai, H D Shen, K Y Chua
JournalInternational archives of allergy and immunology (Int Arch Allergy Immunol) Vol. 121 Issue 3 Pg. 205-10 (Mar 2000) ISSN: 1018-2438 [Print] Switzerland
PMID10729778 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright 2000 S. Karger AG, Basel
Chemical References
  • Allergens
  • Antibodies, Monoclonal
  • Antigens, Dermatophagoides
  • Glycoproteins
  • Immunoglobulin E
Topics
  • Adolescent
  • Adult
  • Allergens (immunology, isolation & purification, metabolism)
  • Antibodies, Monoclonal (biosynthesis, chemistry)
  • Antibody Specificity
  • Antigens, Dermatophagoides
  • Asthma (epidemiology, immunology)
  • Child
  • Female
  • Glycoproteins (immunology, isolation & purification, metabolism)
  • Humans
  • Immunoglobulin E (blood, metabolism)
  • Male
  • Prevalence
  • Singapore (epidemiology)

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