Abstract |
The expression of HLA class I alpha-chain and beta(2)-m subunits was studied at the protein level by a semiquantitative Western blot (WB) approach, in 25 primary breast tumors. The results indicated three pathways of alterations defined comparing the tumor WB gel band with the corresponding PBL gel band: (i) high downregulation pattern (the tumor WB gel band was < or =50% relative to the PBL band), which was found in 44% and 36% of tumors for alpha-chain and beta(2)-m, respectively; (ii) low downregulation pattern (the tumor gel band was between 51% and 75%), which was found in 24% and 20% of tumors for alpha-chain and beta(2)75%), which was found in 32% and 44% of tumors for alpha-chain and beta(2)-m, respectively. The concordance rate with immunohistochemistry (IHC) performed on the same tissue samples was 72% for alpha-chain and 64% for beta(2)-m. This study shows that the use of a semiquantitative WB technique can well define the levels of HLA class I antigens in an autologous setting allowing the biochemical analysis of HLA class I downregulation directly in solid tumor tissues. In addition, the WB technique can be a valuable tool to objectively support the IHC method.
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Authors | M P Pistillo, G Nicolò, S Salvi, P Capanni, L Perdelli, G Pasciucco, G B Ferrara |
Journal | Human immunology
(Hum Immunol)
Vol. 61
Issue 4
Pg. 397-407
(Apr 2000)
ISSN: 0198-8859 [Print] United States |
PMID | 10715517
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- HLA Antigens
- Histocompatibility Antigens Class I
- beta 2-Microglobulin
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Topics |
- Blotting, Western
- Breast Neoplasms
(chemistry, immunology, pathology)
- HLA Antigens
(biosynthesis, chemistry)
- Histocompatibility Antigens Class I
(biosynthesis, chemistry)
- Humans
- Immunohistochemistry
- Staining and Labeling
- beta 2-Microglobulin
(biosynthesis, chemistry)
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