Abstract |
The Polo-like kinase 1 (Plk1) is a highly conserved mitotic serine/threonine kinase which is commonly overexpressed in cancer cell lines. Plk1 positively regulates mitotic progression by activating the CDC25C-CDK1 amplification loop and by regulating late mitotic events, primarily the ubiquitin-dependent proteolysis. In the present study, an antisense strategy against Plk1 mRNA was developed to specifically inhibit cell proliferation of cancer cells in cell culture and in the nude-mouse tumor model. Among 41 phosphorothioate antisense oligodeoxynucleotides tested, the 20-mer JWG2000 strongly inhibited expression of Plk1 in cultured A549 cells, leading to loss of cell viability, and exhibited anti- tumor activity in nude mice A549 xenograft. JWG2000 did not inhibit growth and viability of primary human mesangial cells and human amnion fibroblasts.
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Authors | R Elez, A Piiper, C D Giannini, M Brendel, S Zeuzem |
Journal | Biochemical and biophysical research communications
(Biochem Biophys Res Commun)
Vol. 269
Issue 2
Pg. 352-6
(Mar 16 2000)
ISSN: 0006-291X [Print] United States |
PMID | 10708555
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2000 Academic Press. |
Chemical References |
- Antineoplastic Agents
- Cell Cycle Proteins
- Enzyme Inhibitors
- JWG 2000
- Oligodeoxyribonucleotides
- Oligonucleotides, Antisense
- Protein Kinase Inhibitors
- Proto-Oncogene Proteins
- Thionucleotides
- Protein Kinases
- Protein Serine-Threonine Kinases
- polo-like kinase 1
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Topics |
- Animals
- Antineoplastic Agents
(pharmacology)
- Cell Cycle Proteins
- Cell Division
(drug effects)
- Enzyme Inhibitors
(pharmacology)
- Humans
- Mice
- Mice, Nude
- Oligodeoxyribonucleotides
(pharmacology)
- Oligonucleotides, Antisense
(pharmacology)
- Protein Kinase Inhibitors
- Protein Kinases
- Protein Serine-Threonine Kinases
- Proto-Oncogene Proteins
- Thionucleotides
(pharmacology)
- Tumor Cells, Cultured
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