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Detection and measurement of urinary 2-hydroxyestradiol 17-sulfate, a potential placental antioxidant during pregnancy.

AbstractBACKGROUND:
Preeclampsia is associated with a quantitative imbalance between lipid peroxide and an antioxidant coproduced in the placenta. To investigate our hypothesis that 2-hydroxyestradiol 17-sulfate (2-OH-ES) is the placental antioxidant during pregnancy, we developed an assay for 2-OH-ES in urine and studied samples from women with and without preeclampsia.
METHODS:
The detection and measurement of 2-OH-ES in the urine of pregnant women were performed by RIA using highly specific antiserum to 2-OH-ES. To confirm the reliability of the RIA method, the same samples were analyzed by HPLC using an electrochemical detector.
RESULTS:
Urinary 2-OH-ES values obtained by RIA showed a close relationship to those obtained by HPLC (y = 1.1x - 0.01; r = 0.96). The urinary 2-OH-ES concentrations during the first, second, and third trimesters were 2. 0 +/- 0.6 (mean +/- SE, n = 13), 5.3 +/- 1.3 (n = 21), and 15.3 +/- 2.0 microg/mg creatinine (n = 54), respectively, and <0.15 microg/mg creatinine (n = 10) at 2-24 h after delivery. The concentrations in preeclamptic women during the third trimester were significantly lower, 3.9 +/- 1.9 microg/mg creatinine (mean +/- SE, n = 12).
CONCLUSIONS:
RIA can be used to measure urinary 2-OH-ES during pregnancy. The increase in urinary 2-OH-ES during gestation, its decrease after delivery, and the lower values in preeclampsia are consistent with a role of 2-OH-ES as a placental antioxidant.
AuthorsK Takanashi, T Honma, T Kashiwagi, H Honjo, I Yoshizawa
JournalClinical chemistry (Clin Chem) Vol. 46 Issue 3 Pg. 373-8 (Mar 2000) ISSN: 0009-9147 [Print] England
PMID10702524 (Publication Type: Clinical Trial, Journal Article)
Chemical References
  • Antioxidants
  • Estradiol
  • 2-hydroxyestradiol 17-sulfate
Topics
  • Adolescent
  • Adult
  • Antioxidants (metabolism)
  • Chromatography, High Pressure Liquid
  • Electrochemistry
  • Estradiol (analogs & derivatives, urine)
  • Female
  • Humans
  • Placenta (metabolism)
  • Pre-Eclampsia (urine)
  • Pregnancy
  • Radioimmunoassay

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