Abstract | BACKGROUND: METHODS: The detection and measurement of 2-OH-ES in the urine of pregnant women were performed by RIA using highly specific antiserum to 2-OH-ES. To confirm the reliability of the RIA method, the same samples were analyzed by HPLC using an electrochemical detector. RESULTS: Urinary 2-OH-ES values obtained by RIA showed a close relationship to those obtained by HPLC (y = 1.1x - 0.01; r = 0.96). The urinary 2-OH-ES concentrations during the first, second, and third trimesters were 2. 0 +/- 0.6 (mean +/- SE, n = 13), 5.3 +/- 1.3 (n = 21), and 15.3 +/- 2.0 microg/mg creatinine (n = 54), respectively, and <0.15 microg/mg creatinine (n = 10) at 2-24 h after delivery. The concentrations in preeclamptic women during the third trimester were significantly lower, 3.9 +/- 1.9 microg/mg creatinine (mean +/- SE, n = 12). CONCLUSIONS: RIA can be used to measure urinary 2-OH-ES during pregnancy. The increase in urinary 2-OH-ES during gestation, its decrease after delivery, and the lower values in preeclampsia are consistent with a role of 2-OH-ES as a placental antioxidant.
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Authors | K Takanashi, T Honma, T Kashiwagi, H Honjo, I Yoshizawa |
Journal | Clinical chemistry
(Clin Chem)
Vol. 46
Issue 3
Pg. 373-8
(Mar 2000)
ISSN: 0009-9147 [Print] England |
PMID | 10702524
(Publication Type: Clinical Trial, Journal Article)
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Chemical References |
- Antioxidants
- Estradiol
- 2-hydroxyestradiol 17-sulfate
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Topics |
- Adolescent
- Adult
- Antioxidants
(metabolism)
- Chromatography, High Pressure Liquid
- Electrochemistry
- Estradiol
(analogs & derivatives, urine)
- Female
- Humans
- Placenta
(metabolism)
- Pre-Eclampsia
(urine)
- Pregnancy
- Radioimmunoassay
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