Abstract | OBJECT: METHODS: Sprague-Dawley rats were injected intravenously with either DPL or vehicle. Twenty-four hours later, some animals were tested for superoxide dismutase (SOD) activity. Others were subjected to a 3-hour period of focal cerebral ischemia and, after a reperfusion period of 24 hours, were killed. Infarction volume, SOD activity, and myeloperoxidase (MPO) activity were assayed in the postischemic animals. Pretreatment with DPL produced significant reductions in cerebral infarction and MPO activity in the ischemic penumbra. A significant enhancement of basal SOD activity was observed 24 hours after DPL treatment (that is, before ischemia), and a further enhancement of SOD activity was seen in the ischemic penumbra 24 hours after reperfusion. CONCLUSIONS: These data provide the first evidence of a neuroprotective effect of preconditioning with DPL in an in vivo model of cerebral ischemia. Although the precise mechanisms through which DPL exerts its neuroprotective influence remain to be established, an inhibition of the complex inflammatory response to ischemia and an enhancement of endogenous antioxidant activity are leading candidates.
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Authors | T Toyoda, N F Kassell, K S Lee |
Journal | Journal of neurosurgery
(J Neurosurg)
Vol. 92
Issue 3
Pg. 435-41
(Mar 2000)
ISSN: 0022-3085 [Print] United States |
PMID | 10701530
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Lipid A
- Reactive Oxygen Species
- diphosphoryl lipid A
- Peroxidase
- Superoxide Dismutase
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Topics |
- Animals
- Brain
(blood supply, pathology)
- Cerebral Infarction
(pathology, physiopathology)
- Ischemic Attack, Transient
(pathology, physiopathology)
- Ischemic Preconditioning
(methods)
- Lipid A
(analogs & derivatives, pharmacology)
- Male
- Peroxidase
(metabolism)
- Premedication
- Rats
- Rats, Sprague-Dawley
- Reactive Oxygen Species
(metabolism)
- Reperfusion Injury
(pathology, physiopathology)
- Superoxide Dismutase
(metabolism)
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