Characterization of cytostatically active glycosphingolipids isolated from thioglycollate-elicited murine macrophages.

Abstract

Two acidic glycosphingolipids (gangliosides) derived from mouse macrophage membranes and separated by thin-layer chromatography have a strong cytostatic effect on human and mouse tumor cells. The structure of the two gangliosides, named M phi G1 and M phi G2, was elucidated by application of physicochemical and immunochemical methods. Gas chromatography and mass spectrometry of M phi G1 and M phi G2 classified them as isomeric monosialogangliosides with ceramide moieties composed of sphingosine as the long-chain base, C16 and C18 fatty acids, respectively, and a lacto-tetraose backbone. For M phi G1, additional immunochemical findings led to the proposed structure IV3NeuAc-nLcOse4Cer. The immunochemical reactions of M phi G2 suggest a branched structure for the oligosaccharide moiety.

• Authors: L Schaade, K Ritter, H M Schiebel, R Thomssen, M Kleines
• Journal: IUBMB life (IUBMB Life) Vol. 48 Issue 3 Pg. 353-8 (Sep 1999) ISSN: 1521-6543 [Print] England
• PMID: 10690651 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Glycosphingolipids
• Thioglycolates

Topics

• Animals
• Glycosphingolipids (analysis, metabolism)
• Humans
• Macrophage Activation
• Macrophages, Peritoneal (metabolism)
• Mice
• Thioglycolates