Abstract | UNLABELLED: METHODS: RESULTS: Plasma clearance was rapid, with liver and kidneys representing the major organs of accumulation. No evidence of toxicity, dosage effects, or circulating antiMAG3-EPI-HNE-2 antibodies was observed. Specificity of localization was established using radiolabeled bovine pancreatic trypsin inhibitor (a non-hNE-binding peptide of similar size) as a nonspecific negative control peptide and by predosing with unlabeled EPI-HNE-2 to block receptor sites before the administration of radiolabeled EPI-HNE-2. The ability of radiolabeled EPI-HNE-2 to image inflammation/ infection was evaluated in 12 studies in monkeys receiving only radiolabeled EPI-HNE-2 and with lesions in the arm, shoulder, or lower back. Positive images were obtained in all studies, uptake was apparent almost immediately, and images were still positive 24 h later. As a positive control, animals also received nonspecific IgG antibody radiolabeled with 99mTc either directly or by NHS-MAG3. Compared with labeled antibody, plasma clearance of 99mTc was faster with labeled EPI-HNE-2 and accumulation in liver and heart was lower. Uptake of radioactivity in the inflammation was higher during the first hour with EPI-HNE-2 versus antibody but lower thereafter. CONCLUSION: When radiolabeled with 99mTc, EPI-HNE-2 localized specifically in inflammations in a monkey model and provided early images of diagnostic quality.
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Authors | M Rusckowski, T Qu, J Pullman, R Marcel, A C Ley, R C Ladner, D J Hnatowich |
Journal | Journal of nuclear medicine : official publication, Society of Nuclear Medicine
(J Nucl Med)
Vol. 41
Issue 2
Pg. 363-74
(Feb 2000)
ISSN: 0161-5505 [Print] United States |
PMID | 10688123
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Radiopharmaceuticals
- Serpins
- Technetium Tc 99m Mertiatide
- Leukocyte Elastase
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Topics |
- Animals
- Cattle
- Humans
- Leukocyte Elastase
(antagonists & inhibitors)
- Macaca mulatta
- Radioimmunodetection
- Radiopharmaceuticals
- Serpins
- Staphylococcal Infections
(diagnostic imaging)
- Technetium Tc 99m Mertiatide
- Tissue Distribution
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