HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Tau dephosphorylation at tau-1 site correlates with its association to cell membrane.

Abstract
It has been considered that tau protein is mainly a cytoplasmic protein since it is a microtubule associated protein. However, it has also been suggested that tau could be located in the cell nucleus and membrane. In our work, the cellular distribution of tau has been studied by immunofluorescence and western blot analysis, after subcellular fractionation in neuroblastoma cells and in tau-transfected non neural cells using, mainly, two types of tau antibodies; antibody 7.51 (that recognizes tau independent of its phosphorylation level); and antibody Tau-1 (that recognizes tau only in its dephosphorylated form). Also, tau was expressed in COS-1 cells to test for the features involved in the sorting of tau to different cell localizations. Our results show that tau associated to cell membrane has a lower phosphorylation level in its proline-rich region. Additionally, in differentiated neuroblastoma cells, tau phosphorylation, at that region, decreases and the amount of tau associated to cell membrane increases.
AuthorsM Arrasate, M Pérez, J Avila
JournalNeurochemical research (Neurochem Res) Vol. 25 Issue 1 Pg. 43-50 (Jan 2000) ISSN: 0364-3190 [Print] United States
PMID10685603 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antibodies, Monoclonal
  • tau Proteins
  • tau-1 monoclonal antibody
  • Proline
Topics
  • Animals
  • Antibodies, Monoclonal
  • Binding Sites
  • Blotting, Western
  • COS Cells
  • Cell Fractionation
  • Cell Membrane (metabolism)
  • Cell Nucleus (chemistry)
  • Cytoplasm (chemistry)
  • Fluorescent Antibody Technique
  • Gene Expression
  • Humans
  • Neuroblastoma (metabolism, ultrastructure)
  • Phosphorylation
  • Proline (analysis)
  • Transfection
  • Tumor Cells, Cultured
  • tau Proteins (analysis, genetics, metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: