Abstract |
Tubulin normally undergoes a cycle of detyrosination/tyrosination on the carboxy terminus of its alpha-subunit and this results in subpopulations of tyrosinated tubulin and detyrosinated tubulin. Brain tubulin preparations also contain a third major tubulin subpopulation which is non-tyrosinatable. This review describes the purification and the structural characterization of non-tyrosinatable tubulin. This tubulin variant lacks a carboxyterminal glutamyl- tyrosine group on its alpha-subunit (delta2-tubulin). Delta2-tubulin is generated from detyrosinated tubulin through an irreversible reaction. Delta2-tubulin accumulates in neurons and in stable microtubule assemblies. It also accumulates in some tumor cells due to the frequent loss of tubulin tyrosine ligase in such cells. Delta2-tubulin may be a useful marker of malignancy in human tumors.
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Authors | L Lafanechère, D Job |
Journal | Neurochemical research
(Neurochem Res)
Vol. 25
Issue 1
Pg. 11-8
(Jan 2000)
ISSN: 0364-3190 [Print] United States |
PMID | 10685599
(Publication Type: Journal Article, Review)
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Chemical References |
- Biomarkers, Tumor
- Tubulin
- Tyrosine
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Topics |
- Biomarkers, Tumor
- Humans
- Microtubules
(metabolism)
- Neurons
(metabolism)
- Tubulin
(chemistry, isolation & purification, metabolism)
- Tyrosine
(analysis, chemistry)
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