LOX-1 mediates lysophosphatidylcholine-induced oxidized LDL uptake in smooth muscle cells.

Abstract

A novel receptor for oxidized low-density lipoprotein (OxLDL), lectin-like OxLDL receptor (LOX-1), was cloned from endothelial cells. Since OxLDL is taken up by vascular smooth muscle cells (VSMC) in atheroma, we analyzed the inducible expression of LOX-1 in VSMC in the present study. Incubation of cultured bovine VSMC with lysophosphatidylcholine (LPC), an atherogenic component of OxLDL, increased the level of mRNA for LOX-1 in a dose- and time-dependent manner. Since LPC did not significantly change the half-life of LOX-1 mRNA, the induction seemed to occur at the transcriptional level. The induction accompanied an increase in the protein level of LOX-1 and activity of OxLDL uptake. Blocking antibody against LOX-1 significantly suppressed the enhanced uptake of OxLDL. Thus, LOX-1 is a major receptor for OxLDL in VSMC as in endothelial cells. The enhanced expression of LOX-1 by LPC suggests that OxLDL and LPC would progressively change the function of VSMC and accelerate atherogenesis in vivo.

• Authors: T Aoyama, M Chen, H Fujiwara, T Masaki, T Sawamura
• Journal: FEBS letters (FEBS Lett) Vol. 467 Issue 2-3 Pg. 217-20 (Feb 11 2000) ISSN: 0014-5793 [Print] England
• PMID: 10675541 (Publication Type: Journal Article)

Chemical References

• Lipoproteins, LDL
• Lysophosphatidylcholines
• RNA, Messenger
• Receptors, LDL
• Receptors, Oxidized LDL
• oxidized low density lipoprotein

Topics

• Animals
• Arteriosclerosis (metabolism)
• Cattle
• Cells, Cultured
• Lipoproteins, LDL (metabolism)
• Lysophosphatidylcholines (pharmacology)
• Muscle, Smooth, Vascular (drug effects, metabolism)
• Oxidation-Reduction
• RNA, Messenger (metabolism)
• Receptors, LDL (genetics, metabolism)
• Receptors, Oxidized LDL