Abstract |
To develop an orally delivered subunit vaccine for rotavirus infection, a trypsin cleavage product of VP4, recombinant VP8*, was expressed in Escherichia coli. The recombinant VP8* (rVP8*), purified by affinity chromatography, was reactive against human rotavirus positive serum in Western-blot analysis. To further evaluate the immunogenicity of the oral-delivered rVP8*, it was encapsulated with alginate- microsphere and administered in combination with cholera toxin (CT) as a mucosal adjuvant perorally into mice. The ELISPOT assay showed that the number of rVP8*-specific IgG1 antibody secreting cells increased about 3-fold and about 2-fold in spleen and Peyer's patch, respectively as compared to non-immune mice. In addition, the number of rVP8*-specific IgA antibody secreting cells increased about 2-fold in Peyer's patch. Finally, rVP8*-specific IgA antibody response was significantly enhanced in the intestinal fluids from the mice immunized perorally with encapsulated rVP8* and CT. Taken together, these results indicate that rVP8* possessed proper immunogenicity and it would be potentially useful as a subunit vaccine against rotavirus-associated disease through peroral immunization.
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Authors | D K Kang, P H Kim, E J Ko, J Y Seo, S Y Seong, Y H Kim, I C Kwon, S Y Jeong, J M Yang |
Journal | Molecules and cells
(Mol Cells)
Vol. 9
Issue 6
Pg. 609-16
(Dec 31 1999)
ISSN: 1016-8478 [Print] Korea (South) |
PMID | 10672927
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Adjuvants, Immunologic
- Alginates
- Capsid Proteins
- Immunoglobulin A
- Recombinant Proteins
- VP4 protein, Rotavirus
- Viral Vaccines
- Cholera Toxin
- Trypsin
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Topics |
- Adjuvants, Immunologic
- Administration, Oral
- Alginates
- Animals
- Blotting, Western
- Capsid
(chemistry, immunology)
- Capsid Proteins
- Cholera Toxin
(immunology)
- Drug Compounding
- Enzyme-Linked Immunosorbent Assay
- Escherichia coli
- Humans
- Immunization
- Immunoglobulin A
(immunology)
- Intestinal Mucosa
(immunology)
- Intestines
(immunology)
- Mice
- Microscopy, Electron, Scanning
- Microspheres
- Recombinant Proteins
(immunology)
- Rotavirus Infections
(prevention & control)
- Trypsin
- Viral Vaccines
(administration & dosage)
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