Abstract |
"Self" melanocyte differentiation antigens are potential targets for specific melanoma immunotherapy. Vaccination against murine tyrosinase-related protein (TRP)-1/gp75 was shown recently to cause melanoma rejection, which was accompanied by autoimmune skin depigmentation ( vitiligo). To further explore the linkage between immunotherapy and autoimmunity, we studied the response to vaccination with a related antigen, TRP-2. i.m. inoculation of plasmid DNA encoding murine trp-2 elicited antigen-specific CTLs that recognized the B16 mouse melanoma and protected the mice from challenge with tumor cells. Furthermore, mice bearing established s.c. B16 melanomas rejected the tumor upon vaccination with a recombinant vaccinia virus encoding trp-2. Depletion experiments showed that CD8+ lymphocytes and natural killer cells were crucial for the antitumor activity of the trp-2-encoding vaccines. Mice that rejected the tumor did not develop generalized vitiligo, indicating that protective immunity can be achieved in the absence of widespread autoimmune aggression.
|
Authors | V Bronte, E Apolloni, R Ronca, P Zamboni, W W Overwijk, D R Surman, N P Restifo, P Zanovello |
Journal | Cancer research
(Cancer Res)
Vol. 60
Issue 2
Pg. 253-8
(Jan 15 2000)
ISSN: 0008-5472 [Print] United States |
PMID | 10667570
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Cancer Vaccines
- Interferon Type I
- Peptides
- Pregnancy Proteins
- Vaccines, Synthetic
- interferon tau
- Intramolecular Oxidoreductases
- dopachrome isomerase
|
Topics |
- Animals
- CD8-Positive T-Lymphocytes
(immunology)
- Cancer Vaccines
(therapeutic use, toxicity)
- Interferon Type I
(immunology)
- Intramolecular Oxidoreductases
(immunology)
- Killer Cells, Natural
(immunology)
- Lymphocyte Culture Test, Mixed
- Lymphocyte Depletion
- Melanoma, Experimental
(immunology, therapy)
- Mice
- Mice, Inbred BALB C
- Mice, Inbred C57BL
- Peptides
(chemistry, immunology)
- Pregnancy Proteins
(immunology)
- Vaccines, Synthetic
(therapeutic use, toxicity)
- Vitiligo
(etiology, immunology)
|