Endogenous interleukin-10 regulates Th1 responses that induce crescentic glomerulonephritis.

Abstract

BACKGROUND:

Interleukin (IL)-10 plays a pivotal role in regulating the Th1/Th2 predominance of immune responses. Exogenously administered IL-10 suppresses nephritogenic Th1 responses, inhibits macrophage function, and attenuates crescentic glomerulonephritis (GN). To determine the role of endogenous IL-10, the development of the nephritogenic immune response and crescentic GN was compared in IL-10-deficient (IL-10-/-) and normal (IL-10+/+) C57BL/6 mice.

METHODS:

GN was initiated in sensitized mice by the intravenous administration of sheep antimouse glomerular basement membrane globulin. Renal injury was evaluated 21 days later.

RESULTS:

Following the administration of anti-glomerular basement membrane globulin, normal (IL-10+/+) C57BL/6 mice developed proliferative GN with occasional crescents, glomerular CD4+ T-cell and macrophage accumulation, and fibrin deposition. Using an identical induction protocol, IL-10-/-mice developed more severe GN. Crescent formation (IL-10-/-, 23 +/- 2% of glomeruli; IL-10+/+, 5 +/- 2%), glomerular CD4+ T cells [IL-10-/-, 1. 0 +/- 0.2 cells per glomerular cross-section (c/gcs); IL-10 +/+, 0.3 +/- 0.05 c/gcs], glomerular macrophages (IL-10-/-, 4.8 +/- 0.3 c/gcs; IL-10 +/+, 1.7 +/- 0.2 c/gcs), fibrin deposition [fibrin score (range 0 to 3+); IL-10-/-, 1.10 +/- 0.04; IL-10+/+, 0.6 +/- 0. 07], and serum creatinine (IL-10-/-, 30 +/- 2 micromol/L; IL-10 +/+, 23 +/- 1 micromol/L) were all significantly increased in IL-10-/- mice (P < 0.05). Circulating antibody (IL-10-/-, 1.05 +/- 0.16 OD units; IL-10+/+, 0.63 +/- 0.08 OD units) and cutaneous delayed-type hypersensitivity (skin swelling; IL-10-/-, 0.21 +/- 0.03 mm; IL-10+/+, 0.12 +/- 0.02 mm) to the nephritogenic antigen (sheep globulin) were also increased (both P < 0.05). Interferon-gamma production by cultured splenocytes was increased (IL-10-/- 7.9 +/- 2. 5 ng/4 x 106 cells, IL-10+/+ 0.28 +/- 0.09 ng/4 x 106 cells, P < 0. 05), but IL-4 production was unchanged.

CONCLUSIONS:

Endogenous IL-10 counter-regulates nephritogenic Th1 responses and attenuates crescentic GN.

• Authors: A R Kitching, P G Tipping, J R Timoshanko, S R Holdsworth
• Journal: Kidney international (Kidney Int) Vol. 57 Issue 2 Pg. 518-25 (Feb 2000) ISSN: 0085-2538 [Print] United States
• PMID: 10652028 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies
• Autoantibodies
• Complement C3
• Cytokines
• Immunoglobulins
• antiglomerular basement membrane antibody
• Interleukin-10
• Interferon-gamma
• Fibrin

Topics

• Animals
• Antibodies (immunology)
• Autoantibodies
• Complement C3 (analysis, immunology)
• Cytokines (immunology)
• Female
• Fibrin (immunology)
• Gene Expression (immunology)
• Glomerulonephritis (immunology, pathology)
• Hypersensitivity, Delayed (immunology)
• Immunoglobulins (immunology, pharmacology)
• Interferon-gamma (biosynthesis)
• Interleukin-10 (analysis, genetics, immunology)
• Kidney Glomerulus (immunology, pathology)
• Macrophages (immunology)
• Male
• Mice
• Mice, Inbred C57BL
• Mice, Knockout
• Protein Binding (immunology)
• Sheep
• Species Specificity
• Spleen (cytology, immunology, metabolism)
• Th1 Cells (immunology)