BAG-1, a novel Bcl-2-interacting protein, activates expression of human JC virus.

Abstract	Transcription of the human polyomavirus JC virus (JCV) genome is regulated by cellular proteins and the large tumour (T) antigen. Earlier studies led to the identification of nuclear factor-1 (NF-1)-binding sites in the JCV enhancer by DNase I protection assays of extracts from retinoic acid (RA)-differentiated P19 embryonal carcinoma (EC) cells. In this study, a cDNA clone that encodes a protein capable of binding to the JCV NF-1 sites was isolated from an RA-differentiated EC cell cDNA library. Sequence analysis revealed that the cDNA isolated was identical to the previously described Bcl-2-interacting protein BAG-1 (Bcl-2-associated athano gene-1). Results from RNA studies indicated that BAG-1 is expressed in several cell types. Co-transfection of a recombinant BAG-1 expression plasmid with JCV promoters indicated that BAG-1 stimulates transcription of the JCV(E) promoter and to a lesser extent the JCV(L) promoter. Mutations in the NF-1 sites in the JCV(E) promoter eliminated the activation by BAG-1. Thus, BAG-1 is a novel transcription factor that may play a role in JCV expression.
Authors	L R Devireddy, K U Kumar, M M Pater, A Pater
Journal	The Journal of general virology (J Gen Virol) Vol. 81 Issue Pt 2 Pg. 351-7 (Feb 2000) ISSN: 0022-1317 [Print] England
PMID	10644833 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	BCL2-associated athanogene 1 protein Carrier Proteins DNA, Complementary DNA, Viral DNA-Binding Proteins Neurofibromin 1 Proteins Transcription Factors
Topics	Amino Acid Sequence Base Sequence Binding Sites (genetics) Carrier Proteins (genetics) Cell Line DNA, Complementary (genetics, isolation & purification) DNA, Viral (genetics, isolation & purification) DNA-Binding Proteins Gene Expression Regulation, Viral Genome, Viral HeLa Cells Humans JC Virus (genetics, pathogenicity, physiology) Molecular Sequence Data Neurofibromin 1 Promoter Regions, Genetic Protein Binding Proteins (metabolism) Sequence Homology, Amino Acid Transcription Factors

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