| Abstract | To determine whether interleukin-10 (IL-10) could alter the development of grain dust-induced airway disease, we pretreated mice with either saline or IL-10 intravenously, exposed the mice to an inhalation challenge with corn dust extract (CDE), and measured inflammation and the development of airway hyperreactivity. Pretreatment with IL-10, in comparison to saline, reduced the concentration and percentage of polymorphonuclear cells in the lavage fluid 30 min after the inhalation challenge with CDE (P < 0. 05). In comparison to saline-treated mice, IL-10 did not significantly alter the degree of airway hyperreactivity 30 min after the exposure to CDE. IL-10-treated mice lavaged 18 h after challenge with CDE also exhibited a lower percentage of polymorphonuclear cells in the lavage fluid (P < 0.05) and had significantly less airway hyperreactivity than did mice pretreated with the saline placebo (P < 0.05). These findings indicate that exogenous IL-10 is effective in reducing airway inflammation and airway hyperreactivity due to the inhalation of CDE. |
| Authors | T J Quinn, S Taylor, C L Wohlford-Lenane, D A Schwartz
(Affiliation: Pulmonary, Critical Care, and Occupational Medicine Division, Department of Internal Medicine, University of Iowa, Iowa City, IA 52242, USA.)
|
| Journal | Journal of applied physiology (Bethesda, Md. : 1985)
(J Appl Physiol)
Vol. 88
Issue 1
Pg. 173-9
(Jan 2000)
ISSN: 8750-7587 UNITED STATES |
| PMID | 10642378
(Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
|
| Chemical References |
- Anti-Inflammatory Agents
- Dust
- Lipopolysaccharides
- Monokines
- RNA, Messenger
- Interleukin-10
- Methacholine Chloride
|
| Topics |
- Animals
- Anti-Inflammatory Agents
(administration & dosage, pharmacology, therapeutic use)
- Bronchial Hyperreactivity
(etiology, immunology, prevention & control)
- Bronchoalveolar Lavage Fluid
(chemistry, immunology)
- Dust
(adverse effects)
- Inflammation
(etiology, immunology, prevention & control)
- Interleukin-10
(administration & dosage, pharmacology, therapeutic use)
- Lipopolysaccharides
(administration & dosage, pharmacology)
- Lung
(drug effects, immunology, pathology)
- Lung Diseases, Obstructive
(etiology, immunology, prevention & control)
- Male
- Methacholine Chloride
(diagnostic use)
- Mice
- Mice, Inbred C57BL
- Monokines
(analysis, genetics)
- Neutrophil Infiltration
- RNA, Messenger
(genetics, metabolism)
- Respiratory Tract Diseases
(etiology, immunology, prevention & control)
- Zea mays
|
