Cancer immunomodulation by carbohydrate ligands for the lymphocyte receptor NKR-P1.

Abstract

Natural killer (NK) and T cells express a superfamily of proteins with structural features of C-type lectins. Recombinantly prepared soluble form of rat NKR-P1 (CD161) recognized carbohydrate GalNac and GlcNac moieties. Ganglioside GM2 and heparin related-IS oligosaccharides representing the high affinity ligands for this receptor, increased the sensitivity of targets for killing by the rat effectors isolated from blood and spleen in vitro. Based on these results, we investigated in vivo the possible therapeutic effect of GM2 and IS carried by liposomes during induced rat colorectal carcinogenesis. The reduction of cancer incidence versus the controls (50% vs 88.88%), approached the 5-fluorouracil treatment (41.66%).

• Authors: M Pospisil, L Vannucci, O Horvath, A Fiserova, K Krausova, K Bezouska, F Mosca
• Journal: International journal of oncology (Int J Oncol) Vol. 16 Issue 2 Pg. 267-76 (Feb 2000) ISSN: 1019-6439 [Print] Greece
• PMID: 10639569 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Surface
• Antimetabolites, Antineoplastic
• Carcinogens
• Drug Carriers
• Lectins, C-Type
• Liposomes
• NK Cell Lectin-Like Receptor Subfamily B
• Oligosaccharides
• G(M2) Ganglioside
• Azoxymethane
• Fluorouracil

Topics

• Animals
• Antigens, Surface (drug effects)
• Antimetabolites, Antineoplastic (therapeutic use)
• Azoxymethane
• Carcinogens
• Colorectal Neoplasms (chemically induced, drug therapy)
• Drug Carriers
• Drug Screening Assays, Antitumor
• Escherichia coli
• Fluorouracil (therapeutic use)
• G(M2) Ganglioside (therapeutic use)
• Lectins, C-Type
• Liposomes
• Male
• NK Cell Lectin-Like Receptor Subfamily B
• Oligosaccharides (therapeutic use)
• Rats
• Rats, Sprague-Dawley