The effect of bombesin, cholecystokinin, gastrin, and their antagonists on proliferation of pancreatic cancer cell lines.

Abstract

BACKGROUND:

The role of cholecystokinin (CCK) and gastrin in the development and growth of pancreatic cancer cells is controversial. The aim of this study was to evaluate the role of CCK-8S, gastrin-17, bombesin, and their antagonists on cell lines from patients with pancreatic cancer.

METHODS:

Cell lines were established from pancreatic cancers operated on at our department. The cells were grown in 10% fetal calf serum (FCS). The effects of CCK-8S, gastrin-17, bombesin, and their antagonists in different concentrations and for different time intervals were studied. The cell number was evaluated with the XTT method.

RESULTS:

The cell line LN 36 responded with increased cell number to stimulation by gastrin-17 and decreased cell number to inhibition by the CCK-B receptor antagonist L-365,260. In contrast, LPC 1 responded with increased cell number to CCK-8S and decreased cell number to the CCK-A receptor antagonist devazepide. LPC 2, 6, and 7 were stimulated by CCK-8S, gastrin-17, and their antagonists. LPC 3 showed decreased cell number after inhibition by the antagonists, and LPC 5 and 10 showed increased cell number after stimulation by CCK-8S and gastrin-17. LPC 4 was stimulated by CCK-8S, and LPC 8 was stimulated by all substances except gastrin-17. Intermittent administration of the substances to LN 36 led to a greater effect on the cell number than administration every day, which was not the case with LPC 1 and LPC 3. Bombesin led to an increased growth in LPC 5 but not in LPC 3.

CONCLUSION:

CCK-8S and gastrin-17 led to an increased cell number in some cell lines. A blockade of the CCK-A and CCK-B receptors by their antagonists led to an increased, an unaffected, or a decreased cell number of the cell lines. The effect of bombesin on different cell lines also varied. This shows a great heterogenicity among pancreatic cancer cells from different patients.

• Authors: B Ohlsson, N Fredäng, J Axelson
• Journal: Scandinavian journal of gastroenterology (Scand J Gastroenterol) Vol. 34 Issue 12 Pg. 1224-9 (Dec 1999) ISSN: 0036-5521 [Print] England
• PMID: 10636070 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Benzodiazepinones
• Gastrins
• Phenylurea Compounds
• Receptors, Cholecystokinin
• L 365260
• Cholecystokinin
• Devazepide
• Bombesin

Topics

• Benzodiazepinones (pharmacology)
• Bombesin (antagonists & inhibitors, pharmacology)
• Cell Division (drug effects)
• Cholecystokinin (antagonists & inhibitors, pharmacology)
• Devazepide (pharmacology)
• Dose-Response Relationship, Drug
• Gastrins (antagonists & inhibitors, pharmacology)
• In Vitro Techniques
• Pancreatic Neoplasms (pathology)
• Phenylurea Compounds (pharmacology)
• Receptors, Cholecystokinin (antagonists & inhibitors)
• Tumor Cells, Cultured (drug effects)