Abstract |
Developmental and adult toxicity of musk xylene was studied in Long Evans (LE) rats fed with chow containing musk xylene (MX) in food pellets in concentrations of 1 mg, 10 mg, 33 mg, 100 mg and 1000 mg MX per 1 kg chow corresponding to a daily intake of 0.07-0.08 mg MX/kg up to 70-80 mg MX/kg body weight. Adult male and female rats were MX exposed for a minimum of 10 weeks before mating. Exposure continued throughout pregnancy, birth and lactation. The effects of MX on CYP1A1/1A2 were studied in liver microsomes by EROD (7-ethoxyresorufin-o-deethylase) for CYP1A1 and by MROD ( methoxyresorufin-o-demethylase) for CYP1A2 activity and by Western blotting. MX induced these enzymes dose dependently in adult and developing rats at PN (postnatal day) 1 and 14. The lowest effective maternal dose was 2-3 mg MX/kg/day. Western blot data of CYP2B and CYP3A indicated the induction of both P450 enzyme proteins in developing rats at PN 14 at the higher dose of 70-80 mg MX/kg/day. In contrast, upon high MX exposure CYP2B but not CYP3A was found to be induced in adult first generation male and female rats, indicating differential sensitivity to MX in development.
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Authors | R Suter-Eichenberger, U A Boelsterli, M Conscience-Egli, W Lichtensteiger, M Schlumpf |
Journal | Toxicology letters
(Toxicol Lett)
Vol. 111
Issue 1-2
Pg. 117-32
(Dec 20 1999)
ISSN: 0378-4274 [Print] Netherlands |
PMID | 10630707
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Perfume
- Water Pollutants, Chemical
- Xylenes
- musk xylene
- Cytochrome P-450 Enzyme System
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Topics |
- Administration, Oral
- Animals
- Cytochrome P-450 Enzyme System
(biosynthesis)
- Enzyme Induction
(drug effects)
- Female
- Fetus
(drug effects)
- Male
- Perfume
(toxicity)
- Pregnancy
- Rats
- Rats, Long-Evans
- Water Pollutants, Chemical
(toxicity)
- Xylenes
(toxicity)
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