Effects of intra-articular injections of bufexamac suspension on amphotericin B-induced aseptic arthritis in horses.

Abstract

OBJECTIVE:

To evaluate effects of intra-articular (i.a.) injections of bufexamac on amphotericin B-induced aseptic arthritis in horses.

ANIMALS:

24 Standardbred horses.

PROCEDURE:

Aseptic arthritis was induced in the right intercarpal joint by i.a. injection of amphotericin B (20 mg). One week later (day 0), horses were randomly assigned to four 6-horse treatment groups and treated with i.a. injection of 10, 20, or 40 mg of bufexamac suspension (20 mg/ml) or 2.0 ml of sterile saline (0.9% NaCl) solution (control). The treatment was repeated once after 7 days. Clinical lameness examinations and synovial fluid (SF) analyses were done prior to induction and at weekly intervals for 5 weeks (days 0, 7, 14, 21 and 28).

RESULTS:

Intra-articular injection of amphotericin B consistently resulted in aseptic arthritis with a lameness index (mean +/- SEM; scale 0 to 5) of 2.7 +/- 0.17 on day 0. Intra-articular injections of 20 and 40 mg of bufexamac significantly reduced the day-28 lameness index, compared with control values. Amphotericin B administration also resulted in a significant increase in SF beta-glucuronidase (BGLUC) activity, and i.a. injections of bufexamac significantly reduced day-28 activity of this enzyme, compared with control values.

CONCLUSIONS AND CLINICAL RELEVANCE:

2 i.a. injections of 20 or 40 mg of bufexamac, at weekly intervals, were effective in reducing clinical signs of lameness and SF activity of BGLUC associated with amphotericin B-induced carpal joint arthritis. Bufexamac possesses anti-inflammatory properties useful for i.a. treatment of lameness associated with aseptic arthritis in horses.

• Authors: M M Suominen, R M Tulamo, L M Puupponen, S M Sankari
• Journal: American journal of veterinary research (Am J Vet Res) Vol. 60 Issue 12 Pg. 1467-73 (Dec 1999) ISSN: 0002-9645 [Print] United States
• PMID: 10622153 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• Bufexamac
• Amphotericin B

Topics

• Amphotericin B
• Animals
• Anti-Inflammatory Agents, Non-Steroidal (administration & dosage, therapeutic use)
• Arthritis (chemically induced, veterinary)
• Bufexamac (administration & dosage, therapeutic use)
• Horse Diseases (chemically induced, drug therapy)
• Horses
• Injections, Intra-Articular
• Lameness, Animal (drug therapy)
• Synovial Fluid (chemistry)