Cloning and analysis of cDNAs encoding the hypusine-containing protein eIF5A of two lepidopteran insect species.

Abstract

Eukaryotic initiation factor eIF5A is essential for cell viability and contains a characteristic post-translational modification of a specific lysine residue into a hypusine. cDNAs with similarity to eIF5A sequences were derived from Spodoptera exigua and S. frugiperda cDNA libraries. The deduced amino acid sequences are identical for both species and predict a protein with a molecular mass of 17.5 kDa. The Drosophila melanogaster eIF5A cDNA sequence was retrieved from the Drosophila EST Project. The predicted protein is 80% similar to Spodoptera eIF5A. A single eIF5A gene copy is present in the S. frugiperda genome, which is transcribed into four different transcripts. Infection of S. frugiperda cells with a baculovirus resulted in a strong decline of all four transcripts already at 12 h after infection. In contrast, the eIF5A protein was fairly stable up to 48 h post infection.

• Authors: M M van Oers, M van Marwijk, M S Kwa, J M Vlak, A A Thomas
• Journal: Insect molecular biology (Insect Mol Biol) Vol. 8 Issue 4 Pg. 531-8 (Nov 1999) ISSN: 0962-1075 [Print] England
• PMID: 10620048 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA, Complementary
• Peptide Initiation Factors
• RNA-Binding Proteins
• eukaryotic translation initiation factor 5A
• hypusine
• Lysine

Topics

• Amino Acid Sequence
• Animals
• Baculoviridae (growth & development)
• Base Sequence
• Cloning, Molecular
• DNA, Complementary (genetics)
• Gene Dosage
• Gene Expression
• Lysine (analogs & derivatives)
• Molecular Sequence Data
• Peptide Initiation Factors (genetics)
• Protein Processing, Post-Translational
• RNA-Binding Proteins
• Sequence Analysis, DNA
• Sequence Homology, Amino Acid
• Sequence Homology, Nucleic Acid
• Spodoptera (genetics, virology)