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Cloning and analysis of cDNAs encoding the hypusine-containing protein eIF5A of two lepidopteran insect species.

Abstract
Eukaryotic initiation factor eIF5A is essential for cell viability and contains a characteristic post-translational modification of a specific lysine residue into a hypusine. cDNAs with similarity to eIF5A sequences were derived from Spodoptera exigua and S. frugiperda cDNA libraries. The deduced amino acid sequences are identical for both species and predict a protein with a molecular mass of 17.5 kDa. The Drosophila melanogaster eIF5A cDNA sequence was retrieved from the Drosophila EST Project. The predicted protein is 80% similar to Spodoptera eIF5A. A single eIF5A gene copy is present in the S. frugiperda genome, which is transcribed into four different transcripts. Infection of S. frugiperda cells with a baculovirus resulted in a strong decline of all four transcripts already at 12 h after infection. In contrast, the eIF5A protein was fairly stable up to 48 h post infection.
AuthorsM M van Oers, M van Marwijk, M S Kwa, J M Vlak, A A Thomas
JournalInsect molecular biology (Insect Mol Biol) Vol. 8 Issue 4 Pg. 531-8 (Nov 1999) ISSN: 0962-1075 [Print] ENGLAND
PMID10620048 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • DNA, Complementary
  • Peptide Initiation Factors
  • RNA-Binding Proteins
  • eukaryotic translation initiation factor 5A
  • hypusine
  • Lysine
Topics
  • Amino Acid Sequence
  • Animals
  • Baculoviridae (growth & development)
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary (genetics)
  • Gene Dosage
  • Gene Expression
  • Lysine (analogs & derivatives)
  • Molecular Sequence Data
  • Peptide Initiation Factors (genetics)
  • Protein Processing, Post-Translational
  • RNA-Binding Proteins
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Spodoptera (genetics, virology)

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