Inhibition of experimental autoimmune uveoretinitis with anti-macrophage migration inhibitory factor antibodies.

Abstract

PURPOSE:

The role of macrophage migration inhibitory factor (MIF) in the regulation of ocular autoimmune disease was studied in experimental autoimmune uveoretinitis (EAU) in rats following immunization with a retinal antigen (Ag), interphotoreceptor retinoid-binding protein (IRBP).

METHODS:

LEW rats were immunized with a single injection of IRBP derived peptide, R16(ADGSSWEGVGVVPDV). A neutralizing monoclonal antibody (mAb, IgM) to MIF was injected intraperitoneally every second day from day 0 to day 6 (group A), or from day 8 to day 14 (group B). Control rats were treated with unrelated mouse IgM or PBS. T cell proliferative responses were measured 12 days after immunization. The occurrence and severity of EAU were observed and compared among experimental and control groups.

RESULTS:

T cell proliferative responses against R16 were inhibited in rats treated with anti-MIF mAb compared with the control rats. The development of EAU was delayed in the rats of group A in comparison with those of group B and the control group. The mean histological EAU score on day 18 in group A was 1.11 +/- 0. 11 and significantly lower than those of the group B (1.29 +/- 0.19) and the control (1.67 +/- 0.19).

CONCLUSIONS:

The present result suggests that MIF plays an important role in induction of EAU.

• Authors: N Kitaichi, A Matsuda, S Kotake, K Namba, Y Tagawa, Y Sasamoto, K Ogasawara, K Iwabuchi, K Onoé, H Matsuda, J Nishihira
• Journal: Current eye research (Curr Eye Res) Vol. 20 Issue 2 Pg. 109-14 (Feb 2000) ISSN: 0271-3683 [Print] England
• PMID: 10617911 (Publication Type: Journal Article)

Chemical References

• Antibodies, Monoclonal
• Eye Proteins
• Macrophage Migration-Inhibitory Factors
• Peptide Fragments
• Retinol-Binding Proteins
• interstitial retinol-binding protein

Topics

• Amino Acid Sequence
• Animals
• Antibodies, Monoclonal (immunology, therapeutic use)
• Autoimmune Diseases (chemically induced, prevention & control)
• Eye (drug effects, pathology)
• Eye Proteins
• Injections, Intraperitoneal
• Macrophage Migration-Inhibitory Factors (immunology)
• Male
• Mice
• Mice, Inbred BALB C
• Molecular Sequence Data
• Peptide Fragments (administration & dosage, chemistry)
• Rats
• Rats, Inbred Lew
• Retinitis (chemically induced, prevention & control)
• Retinol-Binding Proteins (chemistry)
• Severity of Illness Index
• T-Lymphocytes (cytology, drug effects, immunology)
• Uveitis (chemically induced, prevention & control)