A comparative study of Celsior and University of Wisconsin solutions based on 12-hr preservation followed by transplantation in canine models.

Abstract

BACKGROUND:

Celsior is a recently developed extracellular-type preservation solution that is effective in organ preservation. This experimental study was designed to compare the effects of Celsior and University of Wisconsin (UW) solutions in myocardial protection, using 12-hour preservation followed by orthotopic transplantation.

METHODS:

Fourteen pairs of adult mongrel dogs were divided into 2 groups. In the UW group (n = 7), UW solution at 4 degrees C was used for coronary vascular washout and storage following cardiac arrest with glucose-insulin-potassium (GIK) solution. In the Celsior group (n = 7), Celsior solution was used to produce cardiac arrest, for coronary vascular washout, and for storage. After 12-hour cold preservation, orthotopic transplantation was performed under cardiopulmonary bypass (CPB). The rate of recovery (%) of cardiac function of donor hearts was compared 1 and 2 hours after weaning from CPB, and then the transplanted hearts were harvested for histological study.

RESULTS:

Hemodynamic parameters including cardiac output, left ventricular pressure (LVP), and the maximum rates of positive and negative increase of LVP after transplantation were significantly (p < 0.05) higher in the Celsior group than in the UW group 2 hours after weaning from CPB. The transmission electron microscopic study found that degeneration of the mitochondria in the Celsior group was less extensive than in the UW group.

CONCLUSION:

Celsior solution enhanced the cardiac function of hearts preserved for 12 hours prior to transplantation compared to UW solution. Our results indicate that Celsior solution is equivalent or superior to UW solution for cardiac preservation.

• Authors: J Mohara, Y Morishita, T Takahashi, K Oshima, T Yamagishi, I Takeyoshi, K Matsumoto
• Journal: The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation (J Heart Lung Transplant) Vol. 18 Issue 12 Pg. 1202-10 (Dec 1999) ISSN: 1053-2498 [Print] United States
• PMID: 10612379 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Celsior
• Disaccharides
• Electrolytes
• Glutamates
• Insulin
• Organ Preservation Solutions
• University of Wisconsin-lactobionate solution
• Mannitol
• Histidine
• Allopurinol
• Glutathione
• Adenosine
• Raffinose

Topics

• Adenosine (pharmacology)
• Allopurinol (pharmacology)
• Animals
• Disaccharides (pharmacology)
• Dogs
• Electrolytes (pharmacology)
• Glutamates (pharmacology)
• Glutathione (pharmacology)
• Heart (drug effects)
• Heart Transplantation
• Hemodynamics (physiology)
• Histidine (pharmacology)
• Insulin (pharmacology)
• Mannitol (pharmacology)
• Myocardium (pathology)
• Organ Preservation (methods)
• Organ Preservation Solutions (pharmacology)
• Raffinose (pharmacology)