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Gamma-heregulin: a fusion gene of DOC-4 and neuregulin-1 derived from a chromosome translocation.

Abstract
gamma-Heregulin was identified as an isoform resulting from alternate splicing of the neuregulin-1 gene, after cloning of its cDNA from the MDA-MB-175 breast cancer cell line. gamma-Heregulin was shown to promote growth of cultured MDA-MB-175 cells resulting from activation of its cognate ErbB tyrosine kinase reporters. We show here that gamma-heregulin is transcribed from a fusion gene resulting from a chromosome translocation in MDA-MB-175 cells. The fusion chromosome is described as dic(8:11)(8qter-->8p12::11q13-->11pter). As a result, the 5' end of the gamma-heregulin gene is derived from the stress-induced gene, DOC-4 (11q13), while the 3' end is from the neuregulin-1 gene (8p12). Thus, expression of gamma-heregulin is under the control of the DOC-4 promoter. By contrast with MDA-MB-175 cells, RT-PCR failed to detect a gamma-heregulin transcript in either E9.5 to E13.5 embryonic mouse tissues, adult mouse tissues or other human tumour cell lines. We conclude, therefore, that gamma-heregulin is not a native isoform of the neuregulin-1 gene, but a novel growth factor that may contribute to tumour cell proliferation.
AuthorsX Liu, E Baker, H J Eyre, G R Sutherland, M Zhou
JournalOncogene (Oncogene) Vol. 18 Issue 50 Pg. 7110-4 (Nov 25 1999) ISSN: 0950-9232 [Print] England
PMID10597312 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Carrier Proteins
  • DNA Primers
  • Neuregulin-1
  • Recombinant Fusion Proteins
  • gamma-heregulin
Topics
  • Base Sequence
  • Carrier Proteins (genetics)
  • Chromosome Mapping
  • Chromosomes, Human, Pair 11
  • Chromosomes, Human, Pair 8
  • Cloning, Molecular
  • DNA Primers
  • Humans
  • In Situ Hybridization, Fluorescence
  • Neuregulin-1 (genetics)
  • Recombinant Fusion Proteins (genetics)
  • Translocation, Genetic
  • Tumor Cells, Cultured

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