Abstract |
gamma-Heregulin was identified as an isoform resulting from alternate splicing of the neuregulin-1 gene, after cloning of its cDNA from the MDA-MB-175 breast cancer cell line. gamma-Heregulin was shown to promote growth of cultured MDA-MB-175 cells resulting from activation of its cognate ErbB tyrosine kinase reporters. We show here that gamma-heregulin is transcribed from a fusion gene resulting from a chromosome translocation in MDA-MB-175 cells. The fusion chromosome is described as dic(8:11)(8qter-->8p12::11q13-->11pter). As a result, the 5' end of the gamma-heregulin gene is derived from the stress-induced gene, DOC-4 (11q13), while the 3' end is from the neuregulin-1 gene (8p12). Thus, expression of gamma-heregulin is under the control of the DOC-4 promoter. By contrast with MDA-MB-175 cells, RT-PCR failed to detect a gamma-heregulin transcript in either E9.5 to E13.5 embryonic mouse tissues, adult mouse tissues or other human tumour cell lines. We conclude, therefore, that gamma-heregulin is not a native isoform of the neuregulin-1 gene, but a novel growth factor that may contribute to tumour cell proliferation.
|
Authors | X Liu, E Baker, H J Eyre, G R Sutherland, M Zhou |
Journal | Oncogene
(Oncogene)
Vol. 18
Issue 50
Pg. 7110-4
(Nov 25 1999)
ISSN: 0950-9232 [Print] England |
PMID | 10597312
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Carrier Proteins
- DNA Primers
- Neuregulin-1
- Recombinant Fusion Proteins
- gamma-heregulin
|
Topics |
- Base Sequence
- Carrier Proteins
(genetics)
- Chromosome Mapping
- Chromosomes, Human, Pair 11
- Chromosomes, Human, Pair 8
- Cloning, Molecular
- DNA Primers
- Humans
- In Situ Hybridization, Fluorescence
- Neuregulin-1
(genetics)
- Recombinant Fusion Proteins
(genetics)
- Translocation, Genetic
- Tumor Cells, Cultured
|