Skin roughness and wrinkle formation induced by repeated application of squalene-monohydroperoxide to the hairless mouse.

Abstract

The present study examines the cumulative effects of sub-erythema application of squalene-monohydroperoxide (Sq-OOH), the initial products of UV-peroxidated squalene, to the skin of hairless mice. Sq-OOH was isolated by the methanol extraction and preparative HPLC method. Repeated topical application of 10 mM Sq-OOH to hairless mice for 3 weeks induced definite skin roughness and crinkle formation. 3-D surface parameter analysis revealed changes in all roughness parameters (number of furrows and crests, distance between a furrow and next crest, and irregularity) of the group treated with more than 3 mM Sq-OOH compared to the control group. These skin surface changes were not induced by squalene, squalene-monohydroxide (Sq-OH) or organic hydroperoxides such as tert-butyl hydroperoxide and cumene-hydroperoxide at 10 mM. Similarly, such changes were not induced by primary irritants, such as sodium lauryl sulfate and n-tetradecane under the same experimental conditions. Skin conductance decreased, following application of 10 mM Sq-OOH. Histological observation revealed that application of 10 mM Sq-OOH induced slight hyperkeratosis, moderate epidermal thickening and slight hyperplasia of sebaceous glands.

• Authors: K Chiba, T Sone, K Kawakami, M Onoue
• Journal: Experimental dermatology (Exp Dermatol) Vol. 8 Issue 6 Pg. 471-9 (Dec 1999) ISSN: 0906-6705 [Print] Denmark
• PMID: 10597136 (Publication Type: Journal Article)

Chemical References

• 2,3-oxidosqualene
• Squalene

Topics

• Administration, Topical
• Animals
• Body Water (drug effects, metabolism)
• Electric Conductivity
• Female
• Mice
• Mice, Hairless
• Skin (drug effects, injuries, pathology)
• Skin Aging (drug effects, pathology, physiology)
• Squalene (administration & dosage, analogs & derivatives, radiation effects, toxicity)
• Ultraviolet Rays (adverse effects)