Abstract | PURPOSE: The aim of this study is to determine if fetal esophageal transplantation can create viable esophageal tissue that may be used for treating long gap esophageal atresia. METHODS: Fetuses of gestational age 19 to 20 days were obtained by hysterotomy of pregnant 15-week-old Lewis rats. A 10-mm long segment of esophagus was obtained from each fetus by thoracolaparotomy and transplanted by wrapping it in a pouch created in the distal omentum of a 5-week-old Lewis rat ( syngeneic transplantation: n = 15). Transplanted fetal esophageal grafts were harvested 10 days post- transplantation and fixed in 10% formalin and embedded in paraffin. H&E was used for histological examination, and PGP 9.5 (a neuronal antibody) was used for immunohistochemistry. Esophageal segments obtained from 10-day-old Lewis rats were used as controls. RESULTS: Thirteen of 15 (87%) grafts were transplanted successfully. The successfully transplanted graft could be mobilized to the thoracic cavity without tension or compromising of vascularity, because of the long omental pedicle. H&E staining and PGP 9.5 immunohistochemistry showed normal esophageal structure with intact esophageal nervous system, comparable with control specimens. CONCLUSIONS: Fetal esophageal transplantation produces viable esophageal tissue that may find application for treating long gap esophageal atresia providing rejection can be controlled adequately.
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Authors | A Yamataka, Y Kato, K Ohshiro, E Miyazaki, K Wang, T Miyano |
Journal | Journal of pediatric surgery
(J Pediatr Surg)
Vol. 34
Issue 11
Pg. 1638-40
(Nov 1999)
ISSN: 0022-3468 [Print] United States |
PMID | 10591559
(Publication Type: Journal Article)
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Topics |
- Animals
- Esophageal Atresia
(mortality, pathology, surgery)
- Esophagus
(pathology, transplantation)
- Female
- Fetal Tissue Transplantation
(methods, mortality)
- Graft Survival
- Immunohistochemistry
- Rats
- Rats, Inbred Lew
- Survival Rate
- Treatment Outcome
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