Colony-promoting activity in mice kidneys with phenylhydrazine hemolytic anemia.

Abstract

Aqueous anemic mice kidney extracts (MKE) were assessed colony-promoting activity (CPA) of hematopoietic progenitor cells in serum-free cultures stimulated by interleukin-3 and erythropoietin (Epo). Mice with hemolytic anemia followed by phenylhydorazine (PHZ) injection for 3 days showed a decrease in the hematocrit (25.4%) and an increase in serum Epo by 14-fold of the control on day 3 after the treatment. At 3 days, the total number of hematopoietic progenitor cells in the bone marrow of PHZ mice decreased by 67% of the control, while these cells in the spleen increased to 22-fold of the control on day 3 and 55-fold on day 6. A significant increase in CPA was observed in MKE prepared from PHZ mice kidneys. Additionally, bone marrow suppressive anemia induced by 5-fluorouracil resulted in enhanced CPA the same as for PHZ mice, but in contrast, anemia with suppression of Epo-production due to nephrotoxicity induced by cisplatin caused a decrease in CPA. These results suggest that CPA in MKE correlates with hematopoietic conditions, and may have a definite role in hematopoiesis through the function of the kidney.

• Authors: T Bamba, I Kashiwakura, M Murakami, M Honda, Y Hayase, Y Takagi
• Journal: Renal failure (Ren Fail) Vol. 21 Issue 6 Pg. 615-25 (Nov 1999) ISSN: 0886-022X [Print] England
• PMID: 10586424 (Publication Type: Journal Article)

Chemical References

• Phenylhydrazines
• phenylhydrazine
• Erythropoietin

Topics

• Anemia, Hemolytic (pathology)
• Animals
• Erythropoietin (blood)
• Hematocrit
• Hematopoiesis
• Hematopoietic Stem Cells (physiology)
• Kidney (cytology)
• Male
• Mice
• Phenylhydrazines
• Spleen (cytology)